Kit Midi C d'ADN circulant HiPure (Protocole sous vide)

HiPure Circulating DNA Midi Kit, designed for efficient purification of short, cell-free DNA (cfDNA) molecules from human biofluids for clinical use:

• Target:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Compatibilité des échantillons:
  • Human plasma
  • Human serum
  • Human urine
  • Samples can be fresh or frozen (once only)
• Focus on Short DNA: The kit isolates short nucleic acids (<1000pb) commonly found in biofluids.
• Efficient Purification: The protocol allows for effective recovery of cfDNA from various biofluids.
• Clinical Applications: The purified cfDNA is suitable for use in in vitro diagnostic (IVD) tests approved for clinical purposes.

Avantages:

• Optimized for Short cfDNA: Specifically designed to capture short DNA fragments relevant for biomarker discovery and liquid biopsies.
• Compatibilité des échantillons larges: Works effectively with various human biofluids, offering flexibility in sample selection.
• Larger Scale Purification: The Midi Kit format allows for processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
• Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.

This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (NIPT), and liquid biopsy analyses, ultimately aiding in patient diagnosis and management.

Caractéristiques

Principe

This product offers a fast and reliable method for purifying DNA using silica column chromatography.

1. Lyse et digestion: The sample is treated with a solution and enzyme to break open cells and degrade proteins, releasing DNA.
2. Binding to Silica: The lysate is transferred to a column. The silica membrane in the column captures DNA molecules, while impurities pass through.
3. Lavage: Wash buffers to remove unbound proteins and cellular debris.
4. Élution: A low-salt buffer elutes the purified DNA from the silica.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.

Avantages

• Haut rendement – processus le plus optimal, ADN libre (>50pb) peut être obtenu dans la mesure maximale
• Haute concentration – faible volume d'élution, assurer une concentration élevée d’acide nucléique
• Haute pureté – méthode à faible liaison alcoolique, éliminant complètement la pollution par les inhibiteurs et les protéines
• Récupération élevée – DNA can be recovered at the level of PG by silica gel column purification

Contenu du kit

Stockage et stabilité

• Keep it cool: Store Proteinase K and Carrier RNA in the fridge (2-8°C) for best results.
• Room temp is okay (for a while): They can last at room temperature (15-25°C) pour jusqu'à 12 semaines sans problèmes.
• Everything else: Store other kit components dry at room temperature (15-25°C), good for at least 18 mois.
• Important: If storing everything cold, pensez à réchauffer les tampons avant utilisation.

Données d'expérimentation