Introduction
- Target: Short DNA fragments (<1000pb) in the blood (tumor markers, fetal DNA) ou de l'urine.
- Efficacité: Purifies circulating nucleic acids from human biofluids (plasma, sérum, urine).
- Applications: Extracted DNA is suitable for approved clinical diagnostic tests.
Avantages:
- Optimized for short DNA analysis.
- Works with various human fluids.
- A larger format processes more samples (potentially higher DNA yield).
- Directly usable in clinical tests.
This Midi Kit empowers researchers and clinical labs for applications in oncology, NIPT, and liquid biopsies.
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolement de l'ADN circulant à partir de 1 à 5 ml de plasma, sérum, and body fluids using vacuum protocol |
| Applications | qPCR, analyse de copeaux liquides ou solides, hybridization SNP detection, etc.. |
| Méthode de purification | Mini colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (vide) |
| Échantillon type | Sérum, plasma, and other cell-free fluid samples |
| Montant de l'échantillon | 1-5ml |
| Volume d'élution | ≥50μl |
| Temps par course | ≤60 minutes |
| Volume de liquide transporté par colonne | 4ml |
| Rendement de liaison de la colonne | 1mg |
Principe
This product offers a streamlined method for isolating DNA using silica column technology:
- Lyse et digestion: The sample undergoes treatment with a special solution (lysat) et une enzyme (protéase) to break open cells and degrade proteins, releasing DNA into the lysate.
- Binding to Silica: Le lysat contenant l'ADN est transféré dans une colonne de silice. The silica membrane in the column selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.
- Lavage: Les tampons de lavage sont passés à travers la colonne, removing unbound proteins and cellular debris that didn’t adhere to the silica.
- Élution: A low-salt buffer is used to elute (release) the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.
Key Advantages:
- Efficient Purification: Silica column technology offers a fast and reliable process for isolating DNA from complex biological samples.
- High-Purity DNA: The selective binding properties of the silica membrane ensure the purification of high-quality DNA, suitable for various downstream applications (RAP, etc.).
- Flux de travail simplifié: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.
This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.
Avantages
- Haut rendement – processus le plus optimal, ADN libre (>50pb) peut être obtenu dans la mesure maximale
- Haute concentration – faible volume d'élution, assurer une concentration élevée d’acide nucléique
- Haute pureté – méthode à faible liaison alcoolique, éliminant complètement la pollution par les inhibiteurs et les protéines
- Récupération élevée – DNA can be recovered at the level of PG by silica gel column purification
Contenu du kit
| Contenu | DIV3182 |
| Temps de purification | 50 |
| Liste de contrôle d'accès tampon | 250 ml |
| Tampon ACB* | 300 ml |
| Tampon DCW1* | 22 ml |
| Tampon DCW2* | 10 ml |
| Protéinase K | 540 mg |
| Tampon de dissolution de protéase | 30 ml |
| ARN porteur | 110 µg |
| Eau sans nucléase | 20 ml |
| Mini-colonnes HiPure CFDNA | 50 |
| 2 Tubes de prélèvement ml | 100 |
| Tube d'extension | 50 |
| Connecteur Vac | 50 |
Stockage et stabilité
- Protéinase K & RNA Carrier: Fridge (2-8°C) for best results.
- Other Kit Components: Température ambiante (15-25°C) is good.
- Storing Cold? Warm buffers up before use.
Données d'expérimentation
Commentaires
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