HiPure Stool DNA Kit

Stool Sample in Molecular Biology Research:

1. Utility and Applications:
   • Stool samples are increasingly utilized in animal molecular genetics, population ecology, behavioral ecology, and intestinal disease diagnosis.
   • They contain gut microbial DNA, food residue sample DNA, and alimentary tract exfoliated cell DNA.
2. Desafíos:
   • Low content of exfoliated cells in the digestive tract and degradation of genetic material pose primary challenges.
   • Presence of inhibitors such as polysaccharides, bile acids, bile salts, bile pigments, digestive juices, and mucus can affect PCR-based molecular analysis.

Importancia de la extracción de ADN:

Critical Role:

• • Selecting appropriate extraction methods is crucial for obtaining high-quality DNA and ensuring successful downstream detection of stool DNA.

HiPure Stool DNA Kit:

1. Solution Provided:
   • The HiPure Stool DNA Kit from Magen Company offers a promising solution.
   • Features good universality, high cost-effectiveness, high yield, and purification.
2. Unique Features:
   • Utilizes a unique solution system and inhibitory factor adsorbent to efficiently remove various impurities in stool samples.
   • The purified DNA can be directly used for PCR, PCR cuantitativa, y otras aplicaciones.
3. Efficiency and Ease of Use:
   • Enables rapid and reliable isolation of high-quality genomic DNA from various stool samples.
   • Capaz de procesar hasta 100 mg of stool samples within 60 minutos.
   • Combines reversible nucleic acid binding properties with columna de giro tecnología, eliminating PCR inhibiting compounds.
   • Suitable for PCR, digestión de restricción, y aplicaciones de secuenciación de próxima generación.
4. Sustainability and Scalability:
   • Eliminates the need for organic extractions, reducing plastic waste and hands-on time.
   • Allows multiple samples to be processed in parallel, enhancing efficiency.

Detalles

Especificaciones

Principio

Stool DNA Extraction Process:

1. Homogeneización y lisis:
   • Stool sample is homogenized and treated in a specially formulated buffer containing detergent.
   • This step effectively lyses bacteria, levadura, and fungal samples present in the stool.
2. Eliminación de contaminantes:
   • Our proprietary Absorber Solution is used to remove humic acid, proteínas, polisacáridos, y otros contaminantes de la muestra lisada.
3. Atacada y lavado:
   • Adjusted binding conditions facilitate the attachment of DNA to a DNA Mini Column.
   • Two rapid wash steps are performed to remove trace contaminants, ensuring purity of the DNA.
4. Elución de ADN purificado:
   • El ADN puro se eluye de la columna en un tampón de resistencia iónica baja.
   • The resulting purified DNA is ready for direct use in downstream applications without the need for further purification.

Ventajas

• Alta pureza – El adsorbente único puede eliminar por completo los factores inhibitorios
• Alta concentración – maximum extraction of total DNA from stool samples
• Alta recuperación – El ADN se puede recuperar a nivel de PG
• Buena repetibilidad – La tecnología de sílice puede obtener resultados ideales en todo momento.

Contenido del kit

Almacenamiento y estabilidad

Instrucciones de almacenamiento:

1. Proteinase K and Buffer PCI:
   • A llegado, almacenar a 2-8 ° C.
   • Almacenamiento a corto plazo (hasta 12 semanas) a temperatura ambiente (15-25°C) no afecta su rendimiento.
2. Componentes del kit restantes:
   • Store at room temperature (15-25°C).
   • Estable por al menos 18 meses en estas condiciones.
3. Entire Kit:
   • Can be stored at 2–8°C.
   • Buffers should be redissolved before use if stored at this temperature.
   • Asegúrese de que todos los buffers estén a temperatura ambiente cuando se usan.