- 產品描述:
- Designed for the purification of high-molecular-weight genomic or mitochondrial DNA.
- Suitable for various blood samples.
- Sample Types:
- 全血
- 巴菲大衣
- 骨髓
- Body fluids
- Purification Time: As little as 25 分分鐘.
- 特徵:
- Convenient and scalable purification procedure.
- Removes contaminants and enzyme inhibitors like proteins and divalent cations.
- Purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of total DNA from whole blood using the economic salt out method |
| 應用領域 | 聚合酶鍊式反應, 酵素消化, Southern hybridization, ETC. |
| 淨化技術 | Salting out method |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | 動物組織 |
| 樣品量 | Unlimited |
| 洗脫體積 | ≥100μl |
| 每次運行時間 | Variation with sample amount |
原則
- Lysis Process:
- Cells are lysed with an anionic detergent in the presence of a DNA stabilizer.
- The DNA stabilizer limits the activity of intracellular DNases and DNases in the environment.
- RNA Removal:
- RNA is eliminated by treatment with an RNA-digesting enzyme.
- 污染物去除:
- Other contaminants, like proteins, are removed by salt precipitation.
- DNA Recovery:
- Genomic DNA is recovered by precipitation with alcohol.
- The recovered DNA is dissolved in Buffer TE.
- Quality Assurance:
- Purified DNA typically exhibits an A260/A280 ratio between 1.7 和 1.9.
- DNA size can reach up to 200 知識庫.
優點
- 安全的 – without phenol-chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic, and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- 高純度 – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
套件內容
| 內容 | D331101 | D331102 | D331103 |
| Purification Volumes | 50 毫升 | 300 毫升 | 1000 毫升 |
| 10 x 緩衝紅血球 | 20 毫升 | 100 毫升 | 3 X 100 毫升 |
| Buffer WTL | 60 毫升 | 350 毫升 | 1000 毫升 |
| Buffer PPS | 20 毫升 | 120 毫升 | 350 毫升 |
| 核糖核酸酶溶液 | 300 微升 | 1.8 毫升 | 6 毫升 |
| 緩衝區TE | 10 毫升 | 30 毫升 | 120 毫升 |
儲存和穩定性
- RNase Solution Storage:
- 抵達時, 儲存於2-8°C.
- 短期儲存 (取決於 24 週) 在室溫下 (15-25℃) 不影響性能.
- 其餘套件組件:
- Dry components can be stored at room temperature (15-25℃).
- 至少穩定 18 在這些條件下幾個月.
- 整個套件存儲:
- 整個套件可在 2-8°C 下保存.
- Buffers should be redissolved before use if stored at this temperature.
- Ensure all buffers are at room temperature when used.
