細菌樣本基因組DNA萃取的前處理 96 樣品

操作說明：

1. 樣本採集:

   • 收集 0.1-1 ml blood sample.

2. 樣品製備:

   • 添加 2-3 times the volume of 1x erythrocyte lysate to the blood sample.
   • Fully invert and mix well.
   • 離心機在 12,000 轉速為 1 分分鐘.
   • Carefully remove the supernatant. The precipitate should be white or light red.
   • 添加 400 μl reagent buffer I and 10 μl proteinase K (10 毫克/毫升) to the precipitate.
   • Vortex and mix for 15 秒, then let it sit at room temperature for 2 分分鐘.

3. Special Case: Low-level Organisms:

   • If processing blood from poultry, birds, or other low-level organisms where red blood cells contain nuclei:
   • No need to add 1x erythrocyte lysate.
   • Directly add 200 μl of reagent buffer I (total volume not exceeding 500 微升).
   • Let it sit at room temperature for 2 分分鐘.

4. Digestion:

   • 添加 10 μl proteinase K (10 毫克/毫升) 到混合物.
   • Thoroughly mix by inversion.
   • Digest at 65°C for 10 分分鐘.
   • Invert and mix 6-7 times during digestion until complete.

5. 轉移至試劑板:

   • Add the lysate from the previous step to the 96-well reagent plate.
   • 按照自動提取方案中概述的具體步驟進行進一步處理.