介紹
This product is specially designed for stool RNA萃取. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-聚合酶鍊式反應 and Northern hybridization.
細節
原則
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
套件內容
| 內容 | R662601 | R662602 | R662603 |
| 淨化時間 | 48 準備工作 | 96 準備工作 | 5 X 96 準備工作 |
| MAGPURE RNA顆粒 | 1.7 毫升 | 4.0 毫升 | 18 毫升 |
| 脫氧核糖核酸酶I | 600 微升 | 2 X 600 微升 | 10 X 600 微升 |
| 脫氧核糖核酸酶緩衝液 | 30 毫升 | 40 毫升 | 200 毫升 |
| Buffer SPL | 30 毫升 | 60 毫升 | 270 毫升 |
| 緩衝phc | 30 毫升 | 60 毫升 | 270 毫升 |
| 緩衝區MCB* | 18 毫升 | 30 毫升 | 150 毫升 |
| 緩衝器 MW1* | 44 毫升 | 66 毫升 | 2 X 220 毫升 |
| 緩衝器 RW2* | 20 毫升 | 50 毫升 | 2 X 100 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 30 毫升 | 120 毫升 |
| 2毫升珠管 | 48 | 96 | 5 X 96 |
儲存和穩定性
到達時，馬式RNA顆粒應在2–8°C下儲存. DNase I 應保存在 -20°C. 然而, 短期儲存 (DNase I 高達 1 週, Magpure RNA顆粒至 8 週) 在室溫下 (15–25°C) 不影響他們的表現. 其餘試劑盒成分可在室溫下保存 (15–25°C) 並且至少穩定 18 months under these
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