介紹
- Extracts DNA from various sources: 組織, 細胞, 血, 唾液, 拭子, 血點, 精液, 和其他臨床樣本.
- Fast and easy to use.
- Purified DNA can be used for 聚合酶鍊式反應, 定量PCR, 南部印跡, viral DNA testing, 和更多.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of total DNA from 1-100ul blood, FFPE, 組織, 和其他樣品 |
| 應用領域 | 第二代測序, 聚合酶鍊式反應, 實時PCR, ETC. |
| 純化方法 | Monodisperse magnetic beads |
| 淨化技術 | 磁珠技術 |
| 工藝方法 | 手動或自動 |
| 樣品類型 | 抗凝血, 濃縮血液, 白膜層, 淋巴球, 和培養細胞 |
| 樣品量 | Body fluid : 10 – 200微升, 組織 : ≤10mg |
| 屈服 | 10的 – 15微克 |
| 洗脫體積 | 80 -100微升 |
| 每次運行時間 |
原則
- 利用高結合磁性顆粒進行純化, ensuring efficient DNA isolation.
- 樣品經歷了裂解和蛋白酶促進的裂解和消化, 將 DNA 釋放到溶液中.
- Magnetic particles and binding solution are introduced, causing DNA to adhere to particle surfaces while impurities such as proteins are left unbound.
- Wash with a specialized solution to remove proteins and impurities, followed by ethanol wash to eliminate salts.
- DNA is ultimately eluted using an Elution Buffer, providing purified DNA ready for downstream applications.
Advantage
- High binding capability – ideal for processing DNA-rich samples like whole blood, 白膜層, and concentrated blood.
- Exceptional purity – features carboxyl magnetic beads with minimal surface adsorption, ensuring superior purity.
- Versatile – applicable across a wide range of clinical samples, offering general utility.
套件內容
| 內容 | IVD3101 |
| 淨化時間 | 200 |
| 結合粒子 | 4.5 毫升 |
| 蛋白酶K | 90 毫克 |
| 蛋白酶溶解緩衝液 | 10 毫升 |
| 緩衝區ATL | 60 毫升 |
| 緩衝液AL | 60 毫升 |
| 緩衝區BD* | 20 毫升 |
| Buffer BXW1* | 110 毫升 |
| 洗脫緩衝液 | 30 毫升 |
儲存和穩定性
- Proteinase K and MagBind Particles should be stored at 2–8°C upon receipt.
- 短期儲存 (取決於 18 週) 在室溫下 (15–25°C) does not compromise their performance.
- The remaining components can be stored dry at room temperature (15–25°C) 至少保持穩定 18 在這些條件下幾個月.
