MagPure A3 Features:
- 技術基礎:
- Utilizes Magen’s solid-phase paramagnetic bead technology for the purification of 聚合酶鍊式反應 擴增子.
- High-Throughput Compatibility:
- Designed for high-throughput applications, facilitating efficient processing of multiple samples.
安培技術:
- 選擇性結合:
- Features an optimized buffer that selectively binds PCR amplicons of 100bp and larger to paramagnetic beads.
- 污染物去除:
- Efficiently removes excess primers, 核苷酸, 鹽類, and enzymes through a simple washing procedure.
- PCR產品的純度:
- Delivers PCR products that are essentially free of contaminants, enhancing downstream application reliability.
細節
規格
| 特徵 | 規格 |
| 主要功能 | 從PCR產物和酶促反應溶液中選擇性恢復DNA (Replace Beckmen or agencourt AmPure) |
| 應用領域 | 定序, gene chip and qPCR, ETC. |
| 淨化技術 | 磁珠技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | PCR products, 酵素反應溶液 |
| 樣品量 | 合適的 |
| 洗脫體積 | ≥10μl |
| 運行時間 | ≤50分鐘 |
原則
Product Purification Method:
- 高結合磁性粒子法:
- 利用高結合磁性顆粒進行純化.
- PCR擴增子結合:
- PCR擴增子與Magpure A3混合.
- 100bp的DNA片段，較大與磁珠結合.
- 污染物去除:
- 多餘的底漆, 核苷酸, 鹽類, and enzymes are removed via a simple washing procedure.
- DNA洗脫:
- 使用洗脫緩衝液或水洗脫純化的DNA.
優點
- Wide application – DNA產品, enzyme digestion system, PCR reaction
- 高回收率 – suitable for 100bp-20kb fragments
- 高純度 – effectively remove dNTP, primer, primer dimer, salt ion and other impurities
- Strong applicability – manual operation or automatic workstation
套件內容
| 內容 | BXP-5 | BXP-50 | BXP-500 |
| MagPure A3 | 5 毫升 | 50 毫升 | 500 毫升 |
儲存和穩定性
存儲和處理說明:
- 儲存條件:
- Store MagPure A3 at 2-8°C upon arrival.
- 穩定 18 在這些條件下幾個月.
- 短期存儲:
- 短期儲存 (取決於 12 週) 在室溫下 (15-25℃) 不影響性能.
- 試劑製備:
- 使用前搖動試劑以確保均勻性.
- The reagent should appear homogenous and consistent.
不要凍結.
評論
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