產品概述
- Suitable for rapid extraction of total DNA from various sources: 組織, 細胞, 血, 唾液, 拭子, 血點, 精液, 和其他臨床樣本.
- Extracted DNA can be used directly for 聚合酶鍊式反應, 定量PCR, 南部印跡, virus DNA testing, 和其他應用.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of total DNA from tissue/blood/body fluid/swab/dry blood spots |
| 應用領域 | 聚合酶鍊式反應, 定量PCR, 南方螺栓和病毒檢測, ETC |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | 組織, 細胞, 血, 唾液, 拭子, 血點, 精液, 和其他臨床樣本 |
| 樣品量 | 實體組織: 1-10毫克; 抗凝血: 200微升 |
| 洗脫體積 | ≥20μl |
| 每次運行時間 | 30 – 60 分分鐘 |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
- Utilizes silica column purification method.
- 用裂解物和蛋白酶裂解和消化樣品, 將DNA釋放到裂解物中.
- Lysate is transferred to an adsorption column.
- 核酸吸附在膜上, while protein is not adsorbed and removed through filtration.
- Proteins and impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10毫米三羥甲基氨基甲烷, 酸鹼度 9.0, 0.5乙二胺四乙酸).
優點
- High-quality DNA – meets a variety of downstream applications, 包括PCR, 定量PCR, 酵素消化, 雜交, ETC.
- 快速地 – without separation of leukocytes, 有機萃取, 或乙醇沉澱
- 簡單的 – all nucleic acids can be obtained by direct digestion
- Wide applicability- handle a variety of liquid samples
套件內容
| 內容 | D301802 | D301803 |
| 淨化時間 | 50 | 250 |
| HiPure DNA 迷你柱 I | 50 | 250 |
| 2毫升收集管 | 100 | 500 |
| 緩衝區ATL | 30 毫升 | 150 毫升 |
| 緩衝液AL | 30 毫升 | 150 毫升 |
| 緩衝器GW1 | 22 毫升 | 88 毫升 |
| 緩衝器GW2 | 12 毫升 | 50 毫升 |
| 蛋白酶K | 24 毫克 | 120 毫克 |
| 蛋白酶溶解緩衝液 | 1.8 毫升 | 10 毫升 |
| 緩衝AE | 15 毫升 | 60 毫升 |
儲存和穩定性
- Proteinase K should initially be stored at 2-8°C upon arrival.
- 室溫短期儲存 (15-25℃) 最多為 12 weeks does not affect its performance.
- 其餘試劑盒成分可在室溫下保存 (15-25℃) 至少保持穩定 18 月.
- 或者, the entire kit can be stored at 2-8°C, but buffers should be redissolved before use.
- Ensure all buffers are at room temperature when used.
實驗數據
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