Blood is a valuable source of DNA for clinical diagnostics and research, but traditional extraction methods present challenges:
- Safety Hazards: Bloodborne pathogens pose a risk of infection during extraction.
- DNA Loss: Red blood cell removal can eliminate desired DNA fractions (病毒性的, circulating DNA).
- Impurities and Inhibitors: Blood components can interfere with downstream applications (聚合酶鍊式反應, 南方印跡).
This product offers a novel DNA extraction solution that addresses these issues:
- Safe and Efficient: No red blood cell removal minimizes infectious waste and contamination risks.
- Comprehensive DNA Recovery: Purifies total DNA (基因組, 線粒體, 病毒性的, circulating) for a complete picture.
- 廣泛的樣品相容性: Works with whole blood (fresh/frozen), 電漿, 血清, 白膜層, 和培養細胞.
- 下游應用程序: Purified DNA is suitable for PCR and Southern blotting.
This innovative method simplifies blood DNA extraction while ensuring safety, efficiency, and comprehensive DNA recovery. It represents a significant advancement for researchers in diagnostics, 法醫, and medical research.
筆記: This bulletin corrects the previous statement about sample compatibility. This method is not suitable for tissue samples.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of total DNA from 2ml blood and 200mg tissue using Midi column |
| 應用領域 | 聚合酶鍊式反應, 南方螺栓和病毒檢測, ETC |
| 純化方法 | Midi 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | 組織, 細胞, 血, 唾液, 拭子, blood spot, 精液, 和其他臨床樣本 |
| 樣品量 | 0.2-2 毫升 |
| 洗脫體積 | ≥300μl |
| 每次運行時間 | ≤80 minutes |
| 每根柱的載液量 | 4毫升 |
| 柱結合率 | 1毫克 |
原則
This product offers a streamlined method for purifying DNA from blood samples using silica column technology.
The Process:
- 裂解和消化: The blood sample is treated with a lysate solution and protease to break open cells and degrade proteins, 將 DNA 釋放到溶液中.
- Binding to Silica: The lysate is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules, 而蛋白質和其他雜質則透過.
- 洗滌: Wash buffers remove unbound proteins and cellular debris that didn’t adhere to the silica.
- 洗脫: 低鹽緩衝液 (通常 10 毫摩爾三羥甲基氨基甲烷, 酸鹼度 9.0, 和 0.5 毫米乙二胺四乙酸) is used to elute the purified DNA from the silica. 這會破壞 DNA 和二氧化矽之間的相互作用, 允許在單獨的管中收集純化的 DNA.
優點
- High-quality DNA – meets a variety of downstream applications, 包括PCR, 定量PCR, 酵素消化, 雜交, ETC.
- 快速地 – without separation of leukocytes, 有機萃取, 或乙醇沉澱
- 簡單的 – all nucleic acids can be obtained by direct digestion
- Wide applicability – handle a variety of liquid samples
套件內容
| 內容 | D311302 | D311303 |
| 淨化時間 | 20 | 100 |
| HiPure gDNA Midi Columns | 20 | 100 |
| 15毫升收集管 | 40 | 200 |
| 緩衝區ATL | 50 毫升 | 250 毫升 |
| 緩衝液AL | 50 毫升 | 250 毫升 |
| 緩衝區GW1* | 22 毫升 | 110 毫升 |
| 緩衝器 GW2* | 12 毫升 | 50 毫升 |
| 核糖核酸酶A | 20 毫克 | 90 毫克 |
| 蛋白酶K | 100 毫克 | 440 毫克 |
| 蛋白酶溶解緩衝液 | 10 毫升 | 30 毫升 |
| 緩衝AE | 20 毫升 | 120 毫升 |
儲存和穩定性
為了獲得最佳性能:
- Store Proteinase K and RNase A at 2-8°C (冰箱) 到達後.
可接受的短期存儲:
- Proteinase K and RNase A can be stored for up to 12 室溫下的幾週 (15-25℃) 不影響他們的表現.
剩餘的套件組件:
- Store all other components of the kit dry at room temperature (15-25℃).
- 他們至少穩定 18 在這些條件下幾個月.
重要提示:
- If storing the entire kit at room temperature, ensure buffers are redissolved before use and always warm all buffers to room temperature before using them.
採購指南
| 姓名 | 貓 | 樣品量 | Leukocyte protocol* | Colum type | 洗脫體積 | Average yield | 每次運行時間 |
| HiPure 血液 DNA 迷你試劑盒 | D3111 | 10-200微升 | 1毫升 | 2ML列 | ≥20μl | 5-9μg/200μl | ≤30分鐘 |
| HiPure 組織&血液 DNA 中型套件 | D3113 | 0.2-2毫升 | 10毫升 | 1.5ML列 | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure 組織&血液 DNA 馬克西套件 | D3115 | 3 -10毫升 | 10毫升 | 15ML列 | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure 組織&血液DNA 96 成套工具 | D3117 | 1-200微升 | 1毫升 | 96 孔板 | 3-8μg/200μl |
