HiPure 組織&血液 DNA Maxi 柱套件

• Challenges in DNA Extraction from Blood Samples:
  1. High Infectious Risk: Blood samples pose a significant infectious hazard to operators and the environment.
  2. Complex DNA Source: Blood DNA contains various types such as mitochondrial, 基因組, and circulating DNA, leading to potential loss during extraction.
  3. Presence of Impurities: Blood samples contain impurities and inhibitory factors that can interfere with DNA extraction and downstream applications.
• Existing Extraction Methods:
  • Phenol-chloroform extraction, salting out, ETC.
  • These methods typically involve pre-treatment steps to remove red blood cells and isolate white blood cells, which may result in loss of nucleic acid information.
• Product Benefits:
  • 快速簡便的淨化: Provides a rapid and straightforward method for total DNA純化.
  • Suitability: Purifies total DNA (基因組, 病毒性的, 線粒體) from whole blood, 組織, 和培養細胞.
  • 應用: Ensures reliable DNA for 聚合酶鍊式反應 and Southern blotting assays.

規格

原則

1. Cell Breakdown: Your sample is lysed (broken open) and digested with enzymes to release DNA into solution.
2. Capture: The mixture is passed through a silica column. DNA sticks to the silica membrane, while proteins and other impurities don’t.
3. Washing Away Impurities: The column is washed to remove any remaining proteins and other unwanted molecules.
4. DNA Release: Pure DNA is eluted (washed off) the silica membrane with a special buffer solution. This purified DNA is then ready for further analysis.

優點

• High-quality DNA – meets a variety of downstream applications, 包括PCR, 定量PCR, 酵素消化, 雜交, ETC.
• 快速地 – without separation of leukocytes, 有機萃取, 或乙醇沉澱
• 簡單的 – all nucleic acids can be obtained by direct digestion
• Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
• Wide applicability – handle a variety of liquid samples
• 經濟 – excellent cost-effectiveness performance

套件內容

儲存和穩定性

Storage Recommendations:

• Proteinase K and RNase A: 抵達時, 儲存於2-8°C. 短期儲存 (取決於 12 週) 在室溫下 (15-25℃) 在不影響性能的情況下可以接受.
• 其餘套件組件: Can be stored at room temperature (15-25℃) 至少保持穩定 18 在這些條件下幾個月.

採購指南