This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. 整個提取過程只有 60-90 分分鐘. DNA可直接用於 聚合酶鍊式反應, 定量PCR, southern Blot, 病毒DNA檢測等.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation total DNA from 50mg simple plant using 96 well bind plate |
| 應用領域 | 聚合酶鍊式反應, SSR, AFLP, RAPD and southern blot, ETC. |
| 純化方法 | 96 孔板 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Economic plant samples |
| 樣品量 | Fresh / frozen plant samples: ≤50mg
Dried plant / seed samples: ≤15mg |
| 洗脫體積 | ≥75μl |
| 每次運行時間 | 30-50 分分鐘 |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. 核糖核酸酶A in the lysis buffer digests the RNA in the sample. 裂解後, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
優點
- High quality DNA – meet a variety of downstream applications, 包括PCR, 定量PCR, 酵素消化, blot hybridization, ETC.
- 高通量 – 96 samples can be processed simultaneously by 96 孔板
- High permeability – using composite membrane technology, no hole plugging
套件內容
| 內容 | D316702 | D316703 |
| 淨化時間 | 4 X 96 準備工作 | 20 X 96 準備工作 |
| 核糖核酸酶溶液 | 5 毫升 | 22 毫升 |
| 洗脫緩衝液 | 120 毫升 | 500 毫升 |
| Buffer SPL | 200 毫升 | 2 X 500 毫升 |
| 緩衝液PS | 100 毫升 | 400 毫升 |
| 緩衝器GW1 | 220 毫升 | 5 X 220 毫升 |
| 緩衝器GW2 | 100 毫升 | 3 X 100 毫升 |
| 1.6毫升收集板 | 4 | 20 |
| HiPure gDNA Plate | 4 | 20 |
| 2.2毫升收集板(DW板) | 8 | 40 |
| 0.8毫升收集板(DW板) | 4 | 20 |
| Sealing Film | 20 | 100 |
儲存和穩定性
RNase solution should be stored at 2-8°C upon arrival. 然而, 短期儲存 (取決於 24 週) 在室溫下 (15-25℃) 不影響其性能. 其餘套件組件可在室溫下乾燥保存 (15-25℃) 並且至少穩定 18 在這些條件下幾個月.
採購指南
| 姓名 | 貓 | Fresh / frozen tissue amount | Dried tissue amount | 列類型 | 洗脫體積 | 屈服 (肌肉) |
| HiPure Plant DNA Mini Kit | D3187 | 150毫克 | 40毫克 | 1.5ML列 | 50 – 100微升 | 3-70微克 |
| HiPure HP 植物 DNA Maxi 試劑盒 | D3163 | 5G | 1G | 50ML列 | 0.7 – 3毫升 | 75-1250微克 |
| HiPure SF Plant DNA Kit | D3164 | 100毫克 | 20毫克 | 1.5ML列 | 50 – 100微升 | 3-50微克 |
| HiPure SF Plant DNA 96 成套工具 | D3167 | 50毫克 | 15毫克 | 96 孔板 | 75 – 150微升 | 3-30微克 |
評論
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