介紹
The HiPure Plant RNA Midi Kit provides fast purification of high-quality RNA from plants, 細胞, 組織, and yeast using silica-membrane spin columns with a binding capacity of 1mg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-聚合酶鍊式反應, 北方印跡, 多聚A+RNA (信使RNA) 純化, 核酸酶保護, 和體外翻譯.
細節
規格
| 特徵 | 規格 |
| 主要功能 | 分離總RNA <1g 簡單植物樣本 (包括含有澱粉的植物樣品) 不含氯仿 |
| 應用領域 | 逆轉錄聚合酶連鎖反應, 定量PCR, 北方雜交, 二代定序, nucleic acid protection, 體外翻譯 |
| 純化方法 | Midi 旋轉柱 |
| 淨化技術 | 二氧化矽技術, DNA filtration technology |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | 植物葉子, roots, stems, fruits, fungi, ETC. |
| 樣品量 | 0.5-1 G |
| 洗脫體積 | ≥400μl |
| 每次運行時間 | ≤40分鐘 |
| 每根柱的載液量 | 4毫升 |
| 柱結合率 | 1毫克 |
原則
The Kit isolates total RNA from up to 1g plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 40 分分鐘. 首先將樣品裂解並均質化. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 400µl water using the Kit.
優點
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- 高品質 – 高純度總RNA可直接用於各種敏感下游應用
- 快速地 – medium isolation of several samples can be completed in 40 分分鐘
- 安全的 – 無需苯酚氯仿萃取
- 敏感的 – RNA can be recovered at the level of PG
- Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
套件內容
| 內容 | R415202 | D415203 |
| 淨化時間 | 20 準備工作 | 100 準備工作 |
| HiPure DNA Midi Column II | 20 | 100 |
| HiPure RNA Midi Columns II | 20 | 100 |
| 15毫升收集管 | 40 | 200 |
| 緩衝RLC | 120 毫升 | 550 毫升 |
| Buffer PRC1 | 120 毫升 | 550 毫升 |
| 緩衝器RW1 | 70 毫升 | 400 毫升 |
| 緩衝器 RW2* | 20 毫升 | 100 毫升 |
| 無核糖核酸酶水 | 30 毫升 | 120 毫升 |
儲存和穩定性
The Kit can be stored dry at room temperature (15-25℃) 並且至少穩定 18 在這些條件下幾個月. 使用時確保所有緩衝液均處於室溫. 裝運期間, crystals or precipitation may form in the Buffer RLC/PRC1. 將緩衝液加熱至 37°C 溶解.
評論
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