HiPure HP Plant RNA Mini Kit combines 胍 isothiocyanate lysing and silica gel membrane purification technology to simplify total RNA萃取. Ultracentrifugation in CsCl purification and LiCl / ethanol precipitation are not required. The kit uses DNase digestion to completely remove DNA. It is suitable for extracting up to 100μg of total RNA (包括miRNA) from plant samples less than 200mg. Several samples can be extracted within 40 分分鐘. The purified RNA can be directly used for RT-聚合酶鍊式反應, fluorescent quantitative RT-PCR, 北方雜交, 二代定序, ETC.
細節
規格
| 特徵 | 規格 |
| 主要功能 | 分離總RNA (包括 miRNA) 從 <200mg 難以萃取的植物樣品 (使用低毒氯仿替代品) |
| 應用領域 | 逆轉錄聚合酶連鎖反應, 定量PCR, 北方雜交, 二代定序, nucleic acid protection, 體外翻譯 |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Hard-to-extraction plant samples such as fruit and seed, grape leaves, tea |
| 樣品量 | ≤200 mg |
| 洗脫體積 | ≥30μl |
| 每次運行時間 | 1-24 samples within 30 分分鐘 |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
This kit uses glass fiber filter membrane purification technique, and only requires simple combination-washing-elution steps. The sample is lysed and homogenized in the solution containing guanidine salt, ethanol is added to provide appropriate binding conditions, and transferred to the purification column for centrifugation. Up to 100µg of RNA can be selectively bound to the membrane, pollutants are efficiently washed off after three times of washing, and finally the purified RNA is eluted by RNase Free Water.
優點
- Completely remove DNA by using of DNase
- 高品質 – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- 快速地 – 可以提取多個樣本 40 分鐘柱法
- 敏感的 – RNA can be recovered at the level of PG
- Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
套件內容
| 內容 | R416502 | D416503 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| HiPure RNA 迷你柱 | 50 | 250 |
| 2毫升收集管 | 100 | 250 |
| 脫氧核糖核酸酶I | 600 微升 | 5 X 600 微升 |
| 脫氧核糖核酸酶緩衝液 | 6 毫升 | 30 毫升 |
| Buffer PAL | 60 毫升 | 270 毫升 |
| Buffer GXP2* | 20 毫升 | 100 毫升 |
| Buffer BDP | 60 毫升 | 270 毫升 |
| 緩衝器RW1 | 50 毫升 | 250 毫升 |
| 緩衝器 RW2* | 20 毫升 | 2 X 50 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 30 毫升 |
儲存和穩定性
DNase I should be stored at -20-8°C upon arrival. 其餘試劑盒成分可在室溫下保存 (15-25℃) 並且至少穩定 18 在這些條件下幾個月. 整個套件可在 2-8°C 下保存, 但在這種情況下，緩衝液應在使用前重新溶解. 使用時確保所有緩衝液均處於室溫.
評論
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