The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, 細胞, 組織, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-聚合酶鍊式反應, 北方印跡, 多聚A+RNA(信使RNA) 純化, nuclease protection and in vitro translation.
細節
規格
| 特徵 | 規格 |
| 主要功能 | 分離總RNA <150 mg fruit or seed using chloroform and Plantzol reagent |
| 應用領域 | 逆轉錄聚合酶連鎖反應, 定量PCR, 北方雜交, Poly A purification, nucleic acid protection and in vitro translation translation |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術, DNA filtration technology |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Simple plant, petal, peel, seed |
| 樣品量 | 50-150毫克 |
| 洗脫體積 | ≥300μl |
| 每次運行時間 | ≤15 minutes（1-24 samples） |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 分分鐘. 首先將樣品裂解並均質化. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.
優點
- 高效 DNA 去除 – 一步 RNA萃取 可有效去除基因組DNA
- 高品質 – 一步RNA萃取試劑結合矽膠柱可獲得最高濃度
- 快速地 – 可以提取多個樣本 15 分鐘柱法
- Wide range – 樣本包括動物, 植物, 細菌, 細胞, ETC
- 高輸出 – large sample amount, and the yield can be up to 200μg
套件內容
| 內容 | R401402 | D401403 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| HiPure RNA 迷你柱 | 50 | 250 |
| 2毫升收集管 | 50 | 250 |
| PlantZol Reagent | 60 毫升 | 200 毫升 |
| Buffer GXP | 30 毫升 | 150 毫升 |
| 緩衝器RW1 | 50 毫升 | 200 毫升 |
| 緩衝器 RW2* | 20 毫升 | 2 X 50 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 30 毫升 |
儲存和穩定性
The Kit can be stored dry at room temperature (15–25°C) 並且至少穩定 18 在這些條件下幾個月. 裝運期間, crystals or precipitation may form in the Buffer RLC/PRC1. 將緩衝液加熱至 37°C 溶解.
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