介紹

The HiPure Fastfilter 質粒DNA Midi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-安全資料表 lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes aspecial filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. 取決於 250 µg high copy number plasmid DNA or 10-75 µg low copy number plasmid DNA can be purified from 15-50 mL overnight culture.

細節

規格

原則

Hipure質粒程序基於細菌細胞的鹼性裂解，然後在高鹽存在下將DNA吸附到二氧化矽上. 套件中使用的獨特二氧化矽膜完全取代了質粒DNA小型玻璃或二氧化矽漿液. 該過程包括 3 基本步驟: Preparationand clearing of a bacterial lysate by alkaline method，然後將上清液轉移到柱中以結合DNA. 洗滌蛋白質及其他雜質後, 核酸最終用低鹽緩衝液洗脫 (10毫米三羥甲基氨基甲烷, 博士學位, 0.5乙二胺四乙酸).

優點

• 高純度 – 純化的質粒可以直接用於測序, 酶消化和PCR, ETC.
• 快速地 – 它只需要 15-60 minutes to complete the isolation
• 高產 – 一列中最多可以粘合70µg質粒

套件內容

儲存和穩定性

套件組件可以在室溫下乾燥 (15-25℃) 並且至少穩定 18 在這些條件下幾個月. 如果緩衝液中形成任何沉澱物, 37℃溫熱溶解. 添加RNase A後，緩衝液P1穩定 6 存儲在2-8°C的月份.

採購指南