Solarbio抗酸桿菌 (空軍基地) 染色套件 (金胺O法)

套件組件

介紹

The cell walls of mycobacteria contain a large amount of lipid on the outside of the peptidoglycan, so it is not easy to stain. When the mycobacterium acid of mycobacteria combines with dye, it is difficult to be destained by acid decolorizing solution. This method is called Acid-Fast Bacillus Stain. Traditional dyeing methods need to promote staining by heating and extending the time. The most representative method is the Ziehl-Neelsen method, which is recommended by the WHO and China Tuberculosis Control Program.

Acid-Fast Bacillus AFB ) 染色套件 ( 金胺O法 ) belongs to the fluorescent staining solution, which needs no heating and is relatively safer than acid-fast hot staining solution. The principle of this method is that after Tauramine o-rhodamine staining and redyeing at room temperature, the acid-fast bacilli show bright yellow, while other bacteria and substances in the background show dark yellow. This method can be used to detect acid-fast bacilli with low magnification.

Self Provide Materials

接種環, 滑動, 蒸餾水, Fluorescence Microscope

協定(/或者 reference only)

1. The sample is to be tested by inoculation ring, coat on the slide, and fixed by
2. AddAuramine O Staining Solution, stain in the dark for 10-15min and wash with
3. Decolorizewith Acid Decolorizing Solution for 2-3min until the smear is not yellow, and wash with
4. Dye for 2min with Redyeing Solution and wash with
5. Gently absorb the water and dry naturally.
6. Microscopic examination under fluorescence

結果

筆記

1. Cover the reagent bottle tightly every time to prevent reagent volatilization and contamination.
2. The fluorescence of auramine o-rhodamine is easy to be attenuated, and the operation should avoid
3. The above reagents are irritant to the human body, please pay attention to the appropriate
4. 為了您的安全與健康, please wear laboratory clothes and disposable