การแนะนำ

This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA คอลัมน์หมุน to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.

รายละเอียด

ข้อมูลจำเพาะ

หลักการ

The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.

ข้อดี

• คุณภาพสูง – high purity total RNA / DNA can be directly used in a variety of downstream applications
• เร็ว – column method can complete the extraction of several samples in 30 นาที
• ปลอดภัย – ไม่จำเป็นต้องสกัดฟีนอลคลอโรฟอร์ม
• Simultaneous extraction- simultaneously isolate DNA and RNA from one sample

เนื้อหาชุด

การจัดเก็บและความเสถียร

HiPure DNA/RNA Kit can be stored dry at room temperature (15-25องศาเซลเซียส) และมีเสถียรภาพเป็นอย่างน้อย 18 เดือนภายใต้เงื่อนไขเหล่านี้. ตรวจสอบให้แน่ใจว่าบัฟเฟอร์ทั้งหมดอยู่ที่อุณหภูมิห้องเมื่อใช้. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.