HiPure DNA Clean Up Kit

HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, พีซีอาร์, การเรียงลำดับ,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.

รายละเอียด

ข้อมูลจำเพาะ

หลักการ

The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

ข้อดี

• High recovery efficiency – จนถึง 80% DNA recovery
• เร็ว – isolation can be completed in 10 minutes by column gel method
• Low elution volume – elution volume can be as low as 10μl
• มีประสิทธิภาพ – efficient removal of various inhibitory factors

เนื้อหาชุด

การจัดเก็บและความเสถียร

HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.