HiPure DNA Clean Up Kit

$60.00$150.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.

Details

Specifications

FeaturesSpecifications
Main FunctionsRecover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution
ApplicationsPCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeCrude DNA, enzymatic reaction solution
Sample amount<span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”><150μl
Recovery80%
Elution volume<span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≥15μl
Time per run<span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤10 minutes
Liquid carrying volume per column800µl
Binding yield of column20µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

  • High recovery efficiency – up to 80% DNA recovery
  • Fast – isolation can be completed in 10 minutes by column gel method
  • Low elution volume – elution volume can be as low as 10μl
  • Efficient – efficient removal of various inhibitory factors

Kit Contents

ContentsD214102D214103
Purification Times100 Preps250 Preps
Buffer GWP30 ml60 ml
Buffer DW220 ml50 ml
Elution Buffer30 ml60 ml
HiPure DNA Mini Columns I50250
2 ml Collection Tubes50250

Storage and Stability

HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.

Additional information

Weight0.65 kg
size

HiPure DNA Clean Up Kit 100 preps, HiPure DNA Clean Up Kit 250 preps

brand name

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