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Solarbio Sucrose-PBS Solution 30%

Name: Solarbio Sucrose-PBS Solution 30%
Brand: Solarbio Life Science
Availability: InStock

$280.00

Доставка в долларах США 45 - Бесплатно свыше долларов США 300

DTE — китайская платформа электронной коммерции, специализирующаяся на онлайн-продажах молекулярных тестов., ИФА, и сопутствующие товары.

Производитель: Ведущие китайские бренды
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Описание

Атрибут	Описание
Кот	G2460
Размер	10*500мл
Хранилище	2-8°С, Годен до 1 месяц
Введение	Phosphate-Buffered Saline (ПБС): Commonly known as PBS Frequently used phosphate buffer solution Consists of: Хлорид натрия Хлорид калия Disodium hydrogen phosphate Potassium dihydrogen phosphate Lacks calcium and magnesium значение pH: 7.4 Commonly employed for: Cell washing during cell culturing or other cell-related procedures Dulbecco’s Phosphate Buffer Solution (D-PBS): Variation of PBS lacking calcium and magnesium Alternative names: Dulbecco’s PBS D-PBSA CMF-DPBS D-PBS DPBS Sucrose-PBS Solution, 30%: Состав: Sucrose and PBS Применение: Precipitates fresh tissue to remove excess water Prevents formation of ice crystals upon freezing Sucrose serves as a dehydrating agent
Протокол (только для справки)	Tissue Preparation: Clean fresh tissue with absolute PBS multiple times. Fix immediately (See Note 1). Dehydration with Sucrose-PBS Solution, 30%: Submerge tissue in 10-20 times its volume of Sucrose-PBS Solution, 30%. Dehydrate at 4°C. Replace sucrose solution every 12-24 часы. Once tissue completely sinks, remove it. Embedding and Further Operations: Proceed with subsequent embedding and other operations as per experimental requirements. Примечание: Fixation of tissues of different sizes: Небольшие образцы (less than 5mm thick) or isolated embryos: Fix with G2161-Neutral Buffered Formalin, 10%, or P1110-Paraformaldehyde Fixative, 4%, для 1 час. Wash with PBS. Large samples (5-20mm thick): Recommended to fix at 4°C for at least 8 часы. Wash with PBS. Extra-large samples (more than 20mm thick): Perfusion fixative recommended. After in-situ fixation, remove samples and wash with PBS. Меры предосторожности: Sucrose solution prone to contamination. Maintain aseptic operations to prevent microbial contamination. Необязательный: Combination with G2461-Sucrose-PBS Solution, 20%, and G2462-Sucrose-PBS Solution, 10%, for complete dehydration while preserving tissue structure. Dehydration Steps: Immerse in sucrose PBS solution sequentially from low to high concentration until completely sinking to the bottom. Safety Measures: Wear experimental clothing and disposable gloves for safety and health.
Примечание	Fixation of Tissues: Small Samples (<5mm thick) or Isolated Embryos: Fix with G2161-Neutral Buffered Formalin, 10%, or P1110-Paraformaldehyde Fixative, 4%, для 1 час. Wash with PBS. Large Samples (5-20mm thick): Fix at 4°C for at least 8 часы. Wash with PBS. Extra-Large Samples (>20mm thick): Perfusion fixative recommended. After in-situ fixation, remove samples and wash with PBS. Меры предосторожности: Sucrose solution prone to contamination. Maintain aseptic operations to prevent microbial contamination. Combination with Sucrose-PBS Solutions: Combine with G2461-Sucrose-PBS Solution, 20%, and G2462-Sucrose-PBS Solution, 10%, for complete dehydration while preserving tissue structure. Dehydration Steps: Immerse in sucrose PBS solution sequentially from low to high concentration until completely sinking to the bottom. Safety Measures: Wear experimental clothing and disposable gloves for safety and health.