Частицы MagPure 100 мл/400 мл/1200 мл/4000 мл

Введение

• Magnetic bead nucleic acid purification technology utilizes nano or micron superparamagnetic material as the matrix, often using black or yellowish-brown ferric oxide.
• The surface of the beads is coated with functional groups such as carboxyl, hydroxyl, или кремниевые группы, which facilitate nucleic acid adsorption.
• Магнитные шарики на основе кремния являются наиболее распространенными., working similarly to classical glass milk or glass fiber filter membrane purification methods.
• Частицы магпур are polydisperse silica magnetic beads, comprising 50% ferric oxide at the core and 50% silica on the surface.
• This product serves various purposes including plasmid extraction, восстановление ДНК гелем, очистка продукта, геномная ДНК и экстракция РНК, as well as viral nucleic acid extraction.

Технические характеристики

Принцип

• Связывание, опосредованное высоким содержанием соли: Magpure particles can selectively recover DNA molecules in solutions containing 2-4M guanidine isothiocyanate, while impurities like protein polysaccharides are not adsorbed.
• Alcohol-mediated binding: Magpure particles can selectively recover DNA/RNA molecules in solutions containing guanidine salt and alcohol (>25%), with proteins and other impurities not being adsorbed.
• After treating biological samples with a digestive solution or lysis buffer, DNA/RNA is released into the reagents from cells, organelles, and protein complexes (рибосомы и нуклеосомы).
• Upon adding Magpure particles and binding solution, DNA/RNA adheres to the surface of the particles, forming the DNA/RNA bead complex.
• The magnetic field then separates and collects the magnetic beads, removing impurities such as protein with the waste liquid.
• Following two or three additional cleaning steps, комплекс магнитных шариков ДНК/РНК ресуспендируют в стерилизованной воде или буфере TE..
• DNA/RNA falls off from the surface of the magnetic beads, achieving purification.

Информация для заказа

Руководство по покупке

• • MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenience of magnetic particles.
  • ДНК связывается с кремнеземной поверхностью магнитных частиц в присутствии хаотропной соли..
  • Bound DNA is efficiently washed, significantly enhancing DNA purity.
  • High-quality DNA is eluted from the magnetic particles.
  • The automated purification procedure removes enzymes, нуклеотиды, and other contaminants and inhibitors completely.
  • Purified DNA is ready for direct use in downstream applications, including sequencing and microarray analysis.