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Экстракция РНК земляники для идентификации шести вирусов с использованием метода мультиплексной ПЦР

Блог / 23/01/2024

Background Strawberries are a type of perennial herbaceous plant in the Rose family (розоцветные) и род Клубника (Фрагария). They […]

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Conclusion Currently, gene expression studies mainly rely on qRT-PCR technology, commonly using reference genes to correct for errors introduced during sample detection. Earlier research generally assumed that housekeeping genes essential for maintaining cellular activities exhibit stable expression patterns under different conditions, being regarded as universal reference genes. However, with the advancement of high-throughput sequencing, more studies suggest that housekeeping genes are not always stable. Optimal reference genes often vary significantly among different species under different conditions. When studying the expression of target genes, using previously published reference genes without validation can lead to biased results. Hence, it's essential to reselect reference genes. In the case of the Cowpea weevil, it was found that the pairwise variation value Vn/n+1 in the GeNorm software's pairwise difference analysis was consistently less than the default value of 0.15 across different developmental stages and various tissues of adult weevils. Therefore, there is no need to include a third reference gene for data correction; the most suitable number of reference genes is two. Comprehensive assessment through △Ct, GeNorm, NormFinder, BestKeeper, and RefFinder online tool suggests using β-Act and β-Tub as internal reference genes for gene expression studies across different developmental stages of the Cowpea weevil. Сходным образом, for various tissues in adult weevils, β-Tub and α-Tub were recommended as internal reference genes. RPL40 was evaluated as relatively suitable for use as one of the internal reference genes in different tissues of adult Cowpea weevils. However, it appeared to be one of the least stable genes among different developmental stages of the weevils. С другой стороны, GAPDH showed the least stability in expression among different tissues of adult Cowpea weevils and was also unsuitable as a reference gene for gene expression studies in different developmental stages of the weevils. Similar results were observed in other Coleopteran insects such as Colaphellus bowringi and Sympiezomias velatus (Tan et al., 2015; Li et al., 2018). This study provides accurate and reliable internal reference genes for accurately analyzing gene expression levels in different developmental stages and various tissues of Однакоpea weevil using qRT-PCR. However, whether these findings apply to other experimental conditions requires further investigation. The expression of internal reference genes in the Cowpea weevil varies significantly under different experimental conditions. This study utilized five methods to evaluate the expression stability of eight candidate internal reference genes in different developmental stages and various tissues of the Cowpea weevil. The results suggest that GeNorm software calculated V2/3 gene pairwise difference values under different conditions, all of which wПоэтому the threshold of 0.15. Therefore, the optimal number of reference genes for studying relative gene expression is two. Конкретно, for different developmental stages of the Cowpea weevil, it is recommended to use β-Act and β-Tub as internal reference genes. Тем временем, for various tissues in adult weevils, β-Tub and α-Tub are recommended as internal reference genes.

Исследования по выбору эталонных генов для флуоресцентной ПЦР в реальном времени в долгоносиках виды

Блог / 08/01/2024

About the research Currently used internal reference genes in qRT-PCR include various genes like ubiquitin-conjugating enzyme (UBC), ribosomal RNA (рРНК),

Исследования по выбору эталонных генов для флуоресцентной ПЦР в реальном времени в долгоносиках виды Читать далее »

определение пола птичьей ДНК

Раскрытие птичьего генома: Тесты ДНК птиц на пол & Мониторинг здоровья (Ключ к нуклеиновой кислоте)

Блог / 28/12/2023

Introduction Determining the gender of birds is important for avian breeders, программы сохранения, и владельцы. Однако, many bird species lack

Раскрытие птичьего генома: Тесты ДНК птиц на пол & Мониторинг здоровья (Ключ к нуклеиновой кислоте) Читать далее »

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