Introduction
- The products undergo rigorous screening and quality control to serve as the primary raw material for nucleic acid extraction.
- Buffer solutions are capable of resisting the impact of strong acids and bases, thereby maintaining a stable pH within the system. It’s crucial to uphold a pH range of 4.0 to 11.0 during nucleic acid extraction.
- Nucleic acids are prone to hydrolysis in acidic conditions but remain stable in neutral or weakly alkaline environments. Tris, or trimethylolaminomethane, functions as a pH buffer within the range of 7.0 to 9.0. Its role is to preserve the stability of nucleic acids post-sample cleavage, preventing degradation and enhancing concentration and purity.
- Tris-containing reagents cannot undergo treatment with DEPC, necessitating rigorous assurance of RNase absence during Tris preparation. Typically, DEPC-treated water is employed for preparation to ensure RNase-free Tris of high purity, exceeding 99.99%.
- This product undergoes stringent testing for RNase and DNase activity, confirming its purity of over 99.99% and absence of DNase or RNase activity.
Details
Specifications
| Features | Specification | |
| Basic content | Recommended application | Nucleic acid extraction |
| CAS Numbers | 1185-53-1 | |
| Formula | C4H11NO3.HCL | |
| Molecular Weight | 157.60 | |
| Stability | Unlimited in dry conditions | |
| Level | Molecular Biology | |
| Appearance | White crystal | |
| Purity | ≥99% | |
| Transportation conditions | Room temperature | |
| Preservation conditions | Room temperature | |
| Impurity parameters | Smell | Nothing |
| Melting point ℃ | 145-155 | |
| Heavymetal | <5 PPM | |
| Losson drying | ≤0.09% | |
| UV absorption value | Absorbance value @ 230 (5%) | ≤0.01 |
| Absorbance value @ 260 (5%) | ≤0.01 | |
| Absorbance value @ 280 (5%) | ≤0.01 | |
| Absorbance value @ 320 (5%) | ≤0.01 | |
| Nucleic acid extraction related | DNase test (0.5M) | Not detected |
| RNase test (0.5M) | Not detected | |
| PH value (2M) | 4.0-5.0 |
Ordering information
| CAT.No. | Product Name | Package |
| C11224 | Tris-HCl, TRIS hydrochloride
(Molecular Biology) |
1KG |
| C11225 | 5KG |





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