Name: Solarbio Sucrose-PBS Solution 30%
Brand: Solarbio Life Science
Availability: InStock

Solarbio Sucrose-PBS Solution 30%

$280.00

Shipping USD 45 - Free over USD 300

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Attribute	Description
Cat	G2460
Size	10*500mL
Storage	2-8°C, valid for 1 month
Introduction	Phosphate-Buffered Saline (PBS): Commonly known as PBS Frequently used phosphate buffer solution Consists of: Sodium chloride Potassium chloride Disodium hydrogen phosphate Potassium dihydrogen phosphate Lacks calcium and magnesium pH value: 7.4 Commonly employed for: Cell washing during cell culturing or other cell-related procedures Dulbecco’s Phosphate Buffer Solution (D-PBS): Variation of PBS lacking calcium and magnesium Alternative names: Dulbecco’s PBS D-PBSA CMF-DPBS D-PBS DPBS Sucrose-PBS Solution, 30%: Composition: Sucrose and PBS Usage: Precipitates fresh tissue to remove excess water Prevents formation of ice crystals upon freezing Sucrose serves as a dehydrating agent
Protocol (for reference only)	Tissue Preparation: Clean fresh tissue with absolute PBS multiple times. Fix immediately (See Note 1). Dehydration with Sucrose-PBS Solution, 30%: Submerge tissue in 10-20 times its volume of Sucrose-PBS Solution, 30%. Dehydrate at 4°C. Replace sucrose solution every 12-24 hours. Once tissue completely sinks, remove it. Embedding and Further Operations: Proceed with subsequent embedding and other operations as per experimental requirements. Note: Fixation of tissues of different sizes: Small samples (less than 5mm thick) or isolated embryos: Fix with G2161-Neutral Buffered Formalin, 10%, or P1110-Paraformaldehyde Fixative, 4%, for 1 hour. Wash with PBS. Large samples (5-20mm thick): Recommended to fix at 4°C for at least 8 hours. Wash with PBS. Extra-large samples (more than 20mm thick): Perfusion fixative recommended. After in-situ fixation, remove samples and wash with PBS. Precautions: Sucrose solution prone to contamination. Maintain aseptic operations to prevent microbial contamination. Optional: Combination with G2461-Sucrose-PBS Solution, 20%, and G2462-Sucrose-PBS Solution, 10%, for complete dehydration while preserving tissue structure. Dehydration Steps: Immerse in sucrose PBS solution sequentially from low to high concentration until completely sinking to the bottom. Safety Measures: Wear experimental clothing and disposable gloves for safety and health.
Note	Fixation of Tissues: Small Samples (<5mm thick) or Isolated Embryos: Fix with G2161-Neutral Buffered Formalin, 10%, or P1110-Paraformaldehyde Fixative, 4%, for 1 hour. Wash with PBS. Large Samples (5-20mm thick): Fix at 4°C for at least 8 hours. Wash with PBS. Extra-Large Samples (>20mm thick): Perfusion fixative recommended. After in-situ fixation, remove samples and wash with PBS. Precautions: Sucrose solution prone to contamination. Maintain aseptic operations to prevent microbial contamination. Combination with Sucrose-PBS Solutions: Combine with G2461-Sucrose-PBS Solution, 20%, and G2462-Sucrose-PBS Solution, 10%, for complete dehydration while preserving tissue structure. Dehydration Steps: Immerse in sucrose PBS solution sequentially from low to high concentration until completely sinking to the bottom. Safety Measures: Wear experimental clothing and disposable gloves for safety and health.