Sucrose-PBS Solution
Sucrose-PBS Solution

Solarbio Sucrose-PBS Solution 30%

$280.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

AttributeDescription
CatG2460
Size10*500mL
Storage2-8°C, valid for 1 month
Introduction
  • Phosphate-Buffered Saline (PBS):
    • Commonly known as PBS
    • Frequently used phosphate buffer solution
    • Consists of:
      • Sodium chloride
      • Potassium chloride
      • Disodium hydrogen phosphate
      • Potassium dihydrogen phosphate
    • Lacks calcium and magnesium
    • pH value: 7.4
    • Commonly employed for:
      • Cell washing during cell culturing or other cell-related procedures
  • Dulbecco’s Phosphate Buffer Solution (D-PBS):
    • Variation of PBS lacking calcium and magnesium
    • Alternative names:
      • Dulbecco’s PBS
      • D-PBSA
      • CMF-DPBS
      • D-PBS
      • DPBS
  • Sucrose-PBS Solution, 30%:
    • Composition:
      • Sucrose and PBS
    • Usage:
      • Precipitates fresh tissue to remove excess water
      • Prevents formation of ice crystals upon freezing
    • Sucrose serves as a dehydrating agent
Protocol (for reference only)
  • Tissue Preparation:
    • Clean fresh tissue with absolute PBS multiple times.
    • Fix immediately (See Note 1).
  • Dehydration with Sucrose-PBS Solution, 30%:
    • Submerge tissue in 10-20 times its volume of Sucrose-PBS Solution, 30%.
    • Dehydrate at 4°C.
    • Replace sucrose solution every 12-24 hours.
    • Once tissue completely sinks, remove it.
  • Embedding and Further Operations:
    • Proceed with subsequent embedding and other operations as per experimental requirements.
  • Note:
    • Fixation of tissues of different sizes:
      • Small samples (less than 5mm thick) or isolated embryos:
        • Fix with G2161-Neutral Buffered Formalin, 10%, or P1110-Paraformaldehyde Fixative, 4%, for 1 hour.
        • Wash with PBS.
      • Large samples (5-20mm thick):
        • Recommended to fix at 4°C for at least 8 hours.
        • Wash with PBS.
      • Extra-large samples (more than 20mm thick):
        • Perfusion fixative recommended.
        • After in-situ fixation, remove samples and wash with PBS.
  • Precautions:
    • Sucrose solution prone to contamination.
    • Maintain aseptic operations to prevent microbial contamination.
  • Optional:
    • Combination with G2461-Sucrose-PBS Solution, 20%, and G2462-Sucrose-PBS Solution, 10%, for complete dehydration while preserving tissue structure.
  • Dehydration Steps:
    • Immerse in sucrose PBS solution sequentially from low to high concentration until completely sinking to the bottom.
  • Safety Measures:
    • Wear experimental clothing and disposable gloves for safety and health.
Note
  • Fixation of Tissues:
    • Small Samples (<5mm thick) or Isolated Embryos:
      • Fix with G2161-Neutral Buffered Formalin, 10%, or P1110-Paraformaldehyde Fixative, 4%, for 1 hour.
      • Wash with PBS.
    • Large Samples (5-20mm thick):
      • Fix at 4°C for at least 8 hours.
      • Wash with PBS.
    • Extra-Large Samples (>20mm thick):
      • Perfusion fixative recommended.
      • After in-situ fixation, remove samples and wash with PBS.
  • Precautions:
    • Sucrose solution prone to contamination.
    • Maintain aseptic operations to prevent microbial contamination.
  • Combination with Sucrose-PBS Solutions:
    • Combine with G2461-Sucrose-PBS Solution, 20%, and G2462-Sucrose-PBS Solution, 10%, for complete dehydration while preserving tissue structure.
  • Dehydration Steps:
    • Immerse in sucrose PBS solution sequentially from low to high concentration until completely sinking to the bottom.
  • Safety Measures:
    • Wear experimental clothing and disposable gloves for safety and health.

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