Introduction
The product is the core raw material for nucleic acid extraction after strict screening and quality control. Sodium dodecyl sulfate (SDS) is a very efficient surfactant, which can dissolve almost all proteins. It can destroy the non-covalent bond of protein, so as to denature the protein and lose its natural conformation and function. In the application of nucleic acid extraction, SDS is often used to destroy the cell membrane (lyse cells) to release soluble substances in cells. It can destroy the connection between protein, protein lipid and lipid lipid, denature the protein structurally and destroy its three-dimensional structure. SDS will precipitate at low temperature because it belongs to the one with the highest critical micelle temperature among detergents, and this precipitation phenomenon will be more obvious in the presence of potassium salt. This characteristic of SDS can be used to remove protein from digestive juice.
Details
Specifications
| Features | Specification | |
| Basic content | CAS Numbers | 151-21-3 |
| Formula | CH3(CH2)11OSO3Na | |
| Molecular Weight | 288.38 | |
| Content | 99.0% | |
| Level | Molecular Biology | |
| Appearance | White flake crystal | |
| Preservation conditions | Room temperature | |
| Stability | Unlimited in dry conditions. Storage in dark container | |
| Impurity parameters | Moisture | ≤1.0% |
| Sodium sulphate | ≤0.1% | |
| Sodium chloride | ≤0.1% | |
| Phosphate | ≤1ppm | |
| Lead | ≤2ppm | |
| Iron | ≤1ppm | |
| Nitrogen | ≤5ppm | |
| Arsenic | ≤0.1ppm | |
| Petroleum ether soluble matter | ≤0.2% | |
| Fraction content | >99.0% | |
| UV absorption value | Absorbance value @ 230 (6M) | ≤0.05 |
| Absorbance value @ 260 (6M) | ≤0.01 | |
| Absorbance value @ 280 (6M) | ≤0.05 | |
| Absorbance value @ 320 (6M) | ≤0.01 | |
| Nucleic acid extraction related | Plasmid extraction test | Adopt |
| Tissue DNA extraction test | Adopt | |
| Circulating DNA extraction | Adopt | |
| DNase test (1%) | Not detected | |
| RNase test (1%) | Not detected | |
| Saturated solution (20%) | It is soluble when stirred at room temperature, No filtering required | |
| PH (1%) | 5.0-8.0 |
Ordering information
| CAT.No. | Product Name | Package |
| C11203 | Sodium dodecyl sulfate (SDS)
(Molecular Biology) |
1KG |
| C11204 | 5KG |
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