Sanshibio Sanshibio TBE (rapid soluble granules)

TBE (rapid soluble granules)

Item No:DA002        Specification：50 Pouches             Storage：RT

Product introduction：

• TBE is a white to off-white instant soluble granule used for preparing 1× TBE buffer.
• Each bag of TBE instant soluble granules can produce 1 L of 1× TBE buffer, offering simplicity and convenience in operation.
• The main components of TBE include Tris, boric acid, and EDTA, with a concentration of 89 mM Tris, 89 mM boric acid, and 2 mM EDTA in 1× TBE buffer.
• TBE serves as a commonly utilized nucleic acid electrophoresis buffer in biology, primarily for DNA agarose gel electrophoresis.
• It exhibits a robust buffering capacity, delivering enhanced separation outcomes for fragments smaller than 1 kb and facilitating longer electrophoresis runs.
• However, when used in agarose gel electrophoresis, TBE may lead to significant electroendosmosis and form non-covalent tetrahydroxyborate complexes with agarose, thereby reducing the recovery of DNA fragments.
• Consequently, it is not recommended for DNA fragment recovery purposes.

Product contents：

Note: The pH value of the 1× buffer solution after preparation is 8.3 ± 0.1 @ 25 °C.

Usage Instructions:

1. Take approximately 600mL of distilled water and add it to a beaker. Place a magnetic stir bar in the beaker.
2. Put the beaker on a magnetic stirrer and slowly add the entire contents of 1 bag of TAE instant granules into the beaker. Stir until completely dissolved.
3. Add distilled water to the TAE solution from step 2 to make up the volume to 1L. This is now the 1× buffer solution (TAE working solution).

Configuration Instructions: