Proteinase K (Lyophilizate, Ultra Pure) with Buffer

$312.00

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DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

  • Proteinase K Characteristics:
    • Stable serine protease with broad substrate specificity.
    • Degrades many proteins in the native state even in the presence of detergents.
    • Not activated by metal ions, chelating agents (e.g., EDTA), sulfhydryl reagents, or trypsin or chymotrypsin inhibitors.
    • Stable over a wide pH range (4-12.5), with optimal activity at pH 6.5–9.5.
    • Activity can be stimulated by denaturing agents (e.g., SDS and Urea).
    • Rapid denaturation occurs at temperatures above 70°C.
    • Autolysis increases at alkaline pH, but some enzyme fragments maintain complete proteolytic activity even after extensive autolysis.
  • Applications:
    • Frequently used in molecular biology to digest unwanted proteins, such as nucleases in DNA or RNA preparations.
    • Typically used at 50–200 μg/ml in nucleic acid preparations.
    • Optimal conditions: pH 7.5-8.0 and 37-55°C.
    • Incubation times vary from 30 minutes to 18 hours.

Specifications

FeaturesSpecifications
Molecular Weight29.3kDa
Isoelectric Point8.9
AppearanceWhite lyophilized powder
Purity95% (SDS-PAGE analysis)
Specific activity≥34 Units/mg protein
Temperature characteristicThe effective activity temperature is 37-70°C, and the enzyme activity at 65°C is twice that at 25°C.
PH characteristics4.0-12.0, the optimum range is ph7.5-11.5
Preservation ConditionsIt is recommended to store at -20°C to ensure its stability to the greatest extent (normal temperature transportation or storage will not reduce enzyme activity).

The shelf life is up to 3 years at -20°C and 2 years at 2~8°C.

Usage methodPrepare 20mg/ml with the solution, add proteinase K to the digestive solution or lysate until the final concentration is 50-200ug/ml, and incubate at 55~70°C.

Proteinase K after reaction can be removed or inactivated by magnetic bead method, column method or phenol-chloroform extraction. Protease K can be inactivated by incubation at 95°C for 3 minutes or 70°C for 15 minutes.

Nucleic acid residue detectionQubit did not detect

Human DNA contamination not detected (real-time PCR)

Bacterial DNA contamination not detected (16S universal primer PCR, 30 cycles)

Fungal DNA contamination not detected (ITS Primer PCR)

Nuclease detectionDNase not detected

RNase not detected

Nickase not detected

Recommended applicationNucleic acid extraction, circulating DNA extraction, virus nucleic acid extraction

Advantages

  • Not including RNase, DNase, and Nickase
  • No nucleic acid residue (not detected by Qubit after extracting the whole 100mg protein K)
  • No human-source DNA contamination (not detected by Q-PCR)
  • No bacterial DNA contamination (not detected by 16S universal primer)
  • No fungal DNA contamination (not detected by 1TS universal primer)
  • Can be transported and valid for two years at normal temperature in a dry environment.
  • Can be used for highly sensitive free DNA extraction, virus nucleic acid extraction, whole blood DNA extraction, etc.

Ordering information

ContentsC12100C12101C12102PDB-1000
Proteinase K, Lyophilizate, >30 units/mg of protein1 g10 g100 g
Buffer PDB (20mM Tris, pH 7.5, 10mM CaCl2, 50% glycerol, 0.1% Preservatives)1000 ml

Additional information

Weight0.75 kg

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