Pre-processing of bacterial sample genomic DNA extraction 96 Samples

$225.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

 Instruction:

  1. Sample Collection:

    • Collect 0.1-1 ml blood sample.
  2. Sample Preparation:

    • Add 2-3 times the volume of 1x erythrocyte lysate to the blood sample.
    • Fully invert and mix well.
    • Centrifuge at 12,000 rpm for 1 minute.
    • Carefully remove the supernatant. The precipitate should be white or light red.
    • Add 400 μl reagent buffer I and 10 μl proteinase K (10 mg/ml) to the precipitate.
    • Vortex and mix for 15 seconds, then let it sit at room temperature for 2 minutes.
  3. Special Case: Low-level Organisms:

    • If processing blood from poultry, birds, or other low-level organisms where red blood cells contain nuclei:
    • No need to add 1x erythrocyte lysate.
    • Directly add 200 μl of reagent buffer I (total volume not exceeding 500 μl).
    • Let it sit at room temperature for 2 minutes.
  4. Digestion:

    • Add 10 μl proteinase K (10 mg/ml) to the mixture.
    • Thoroughly mix by inversion.
    • Digest at 65°C for 10 minutes.
    • Invert and mix 6-7 times during digestion until complete.
  5. Transfer to Reagent Plate:

    • Add the lysate from the previous step to the 96-well reagent plate.
    • Follow specific steps outlined in the automatic extraction protocol for further processing.

Additional information

Weight0.7 kg
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