HiPure Soil RNA Kit

$310.00$1,343.00

Shipping USD 45 - Free over USD 300

Isolation total RNA from 500 mg soil sample

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  • Eligible for return or replacement within 30 days
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Description

Introduction

HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total RNA from 500 mg soil sample
ApplicationsRT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeForest soil, grassland soil, mining area soil, sediment and other samples
Sample amount500 mg
Elution volume≥30μl
Time per run≤60 minutes
Liquid carrying volume per column800µl
Binding yield of column100µg

Principle

The hipure silica gel column is based on a glass fiber filter membrane with a high binding force. Under the condition of a high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonds and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

Advantages

  • High quality – high purity total RNA can be directly used in various sensitive downstream applications
  • Fast – extraction of several samples can be completed in 60 minutes by the column purification method
  • Safe – no phenol-chloroform extraction required
  • Sensitive – RNA can be purified at the level of PG

Kit Contents

ContentsR418302R418303
Purification Times50 Preps250 Preps
HiPure RNA Micro Columns50250
2ml Collection Tubes100500
gDNA Filter Column50250
2ml Beads Tubes50250
Buffer SOL30 ml150 ml
Buffer SDS4 ml15 ml
Buffer PHC30 ml150 ml
Buffer GDP40 ml150 ml
Buffer RW150 ml200 ml
Buffer RW2 *20 ml2 x 50 ml
RNase Free Water10 ml30 ml

Storage and Stability

Buffer PHC should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case, buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

R41832.jpg

Additional information

Weight0.85 kg
size

HiPure Soil RNA Kit 50 preps, HiPure Soil RNA Kit 250 preps

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