HiPure Circulating DNA Kit IVD 3182

$547.00

Shipping USD 45 - Free over USD 300

Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol

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  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
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Description

Introduction

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. The extracted products can be used for clinical in vitro detection.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol
ApplicationsqPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (vacuum)
Sample typeSerum, plasma and other cell-free fluid samples
Sample amount1-5ml
Elution volume≥50μl
Time per run≤60 minutes
Liquid carrying volume per column4ml
Binding yield of column1mg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer.

Advantages

  • High yield – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
  • High concentration – low elution volume, ensuring high nucleic acid concentration
  • High purity – low alcohol binding method, completely removing inhibitor and protein pollution
  • High recovery – DNA can be recovered at thelevel of PG by silica gel column purification

Kit Contents

ContentsIVD3182
Purification Times50
Buffer ACL250 ml
Buffer ACB*300 ml
Buffer DCW1*22 ml
Buffer DCW2*10 ml
Proteinase K540 mg
Protease Dissolve Buffer30 ml
Carrier RNA110 μg
Nuclease Free Water20 ml
HiPure CFDNA Mini Columns50
2 ml Collection Tubes100
Extender Tube50
Vac-Connector50

Storage and Stability

Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

Additional information

Weight0.75 kg

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