Product Introduction
The quantitative polymerase chain reaction (qPCR) is an established method for the highly sensitive detection and quantification of DNA or RNA. The measuring principle is based on fluorescent signals, which, cycle by cycle, capture the presence of the existing target sequence in real-time. The key features of this detection method are outstanding high-performance optics as well as excellent temperature uniformity over 96 samples.
The Archimed product family guarantees well-founded real-time PCR results as it benefits from peerless temperature control precision in the sample block regardless of the number of samples used. The patented high-performance optics guarantee the outstanding homogeneous excitation illumination of all individual samples. The theArchimed product family sets new standards of flexibility and precision -for all real-time PCR applications.
Technical Specifications
| Thermal Cycler | Optical Detection | ||
| Block capacity | 96 | Excitation source | Long-life, high-performance LEDs |
| Sample volume | 10-50l | Detector | Highly sensitive MPPC with Fresnel lens |
| Heating/cooling method | Peltier | Scanning principle | Time-resolved scanning technology |
| Maximum block ramp rate | 6℃s | Detector position | Top of the block |
| Temperature setting range | 4-100℃ | Excitation/detection range | 455-650nm/510-715nm |
| Heated lid | Electronic automatic lid | Fluorescence channel | 4 channels(Archimed X4) 6 channels(Archimed X6) |
| Temperature accuracy | ±0.2℃ | Detection sensitivity | 1 copy of the target sequence |
| Temperature uniformity | ±0.2℃ | System sensitivity | Detect differences as small as 1.33-fold in target quantities in singleplex reactions |
| Gradient zone | 12 columns | Dynamic range | 10 orders of magnitude |
| Gradient range | 1-36C | Dye compatibility | FAM/SYBR Green, VIC/JOEHEX/TET, NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LZ |
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