| Attribute | Details |
|---|---|
| Cat | G1571 |
| Size | 10mL |
| Storage | 2-8°C, avoid light, valid for 6 months |
| Introduction | Janus Green B, also known as Janus Green, is a vital dye specifically utilized for staining mitochondria. The principle of staining involves its interaction with cytochrome c oxidase within mitochondria, resulting in a blue-green coloration. Janus Green B (0.2%) solution comprises Janus Green B, salt, distilled water, etc. It is imperative to maintain the material’s activity and work swiftly during the staining process. |
| Protocol | Follow the specific requirements of the experiment. Mix freshly extracted mitochondria with Janus Green B Stain Solution (0.2%) in equal amounts and stain for 1-3 minutes. Dyeing time may vary depending on the sample. |
| Note | Stained mitochondria should be freshly extracted and stained promptly. For safety and health purposes, wear appropriate experimental attire and disposable gloves. |
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