- 제품 설명:
- Designed for the purification of high-molecular-weight genomic or mitochondrial DNA.
- Suitable for various blood samples.
- 샘플 유형:
- 전혈
- 버피 코트
- 골수
- Body fluids
- Purification Time: As little as 25 분.
- 특징:
- Convenient and scalable purification procedure.
- Removes contaminants and enzyme inhibitors like proteins and divalent cations.
- Purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
명세서
| 특징 | 명세서 |
| 주요 기능 | Isolation of total DNA from whole blood using the economic salt out method |
| 응용 | PCR, 효소 소화, Southern hybridization, 등. |
| 정제기술 | Salting out method |
| 가공방법 | 수동 (원심분리 또는 진공) |
| 샘플 유형 | 동물 조직 |
| 샘플량 | Unlimited |
| 용출량 | ≥100μl |
| 실행당 시간 | Variation with sample amount |
원칙
- Lysis Process:
- Cells are lysed with an anionic detergent in the presence of a DNA stabilizer.
- The DNA stabilizer limits the activity of intracellular DNases and DNases in the environment.
- RNA Removal:
- RNA is eliminated by treatment with an RNA-digesting enzyme.
- 오염물질 제거:
- Other contaminants, like proteins, are removed by salt precipitation.
- DNA Recovery:
- Genomic DNA is recovered by precipitation with alcohol.
- The recovered DNA is dissolved in Buffer TE.
- Quality Assurance:
- Purified DNA typically exhibits an A260/A280 ratio between 1.7 그리고 1.9.
- DNA size can reach up to 200 kb.
장점
- 안전한 – without phenol-chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic, and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- 고순도 – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
키트 내용물
| 내용물 | D331101 | D331102 | D331103 |
| Purification Volumes | 50 밀리리터 | 300 밀리리터 | 1000 밀리리터 |
| 10 X 버퍼 RBC | 20 밀리리터 | 100 밀리리터 | 3 엑스 100 밀리리터 |
| Buffer WTL | 60 밀리리터 | 350 밀리리터 | 1000 밀리리터 |
| Buffer PPS | 20 밀리리터 | 120 밀리리터 | 350 밀리리터 |
| RNase 솔루션 | 300 μl | 1.8 밀리리터 | 6 밀리리터 |
| Buffer TE | 10 밀리리터 | 30 밀리리터 | 120 밀리리터 |
보관 및 안정성
- RNase Solution Storage:
- 도착 시, 2~8°C에서 보관.
- 단기 보관 (까지 24 주) 실온에서 (15-25℃) 성능에 영향을 미치지 않습니다.
- 나머지 키트 구성 요소:
- Dry components can be stored at room temperature (15-25℃).
- 적어도 안정적입니다 18 이런 조건에서 몇 달 동안.
- 전체 키트 저장:
- 전체 키트는 2~8°C에서 보관할 수 있습니다..
- Buffers should be redissolved before use if stored at this temperature.
- Ensure all buffers are at room temperature when used.
