소개

This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA 스핀 컬럼 to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.

세부

명세서

원칙

The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. 샘플은 먼저 용해 및 균질화됩니다. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.

장점

• 고품질 – high purity total RNA / DNA can be directly used in a variety of downstream applications
• 빠른 – column method can complete the extraction of several samples in 30 분
• 안전한 – 페놀 클로로포름 추출이 필요하지 않습니다
• Simultaneous extraction- simultaneously isolate DNA and RNA from one sample

키트 내용물

보관 및 안정성

HiPure DNA/RNA Kit can be stored dry at room temperature (15-25°C) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안.