ソーラーバイオダイレクトビリルビン (DBIL) アッセイキット50T | 48S

注記: テスト前に予測のために 2 つまたは 3 つの異なるサンプルを採取します.

操作装置: 分光光度計/マイクロプレートリーダー

カタログ番号: BC5175

サイズ: 50T | 48S

コンポーネント:

試薬I: 25 mL×1. Store at 2-8℃.

試薬II: 6 mL×1. Store at 2-8℃.

製品説明：

直接ビリルビン (DBIL) is also called conjugated bilirubin. After indirect bilirubin enters the liver, it is combined with glucuronic acid by the action of glucuronosyltransferase in the liver. The increase of direct bilirubin is of great significance for clinical diagnosis of obstructive jaundice, hepatocellular jaundice, liver cancer, pancreatic head cancer, cholelithiasis, and cholangiocarcinoma. Direct bilirubin could be oxidized by sodium nitrite to form biliverdin, which has an absorbance of 450 nm. The content of direct bilirubin can be calculated by detecting the wavelength change at 450 nm.

必要だが提供されていない試薬と装置：

分光光度計/マイクロプレートリーダー, 卓上遠心分離機, constant temperature foster box/water bath, pipette, マイクロガラスキュベット/96ウェル平底プレート, 氷, と蒸留水.

手順

あ. サンプルの準備:

血清, プラズマ, or other liquid samples: Detect the sample directly. If the solution is turbid, measure centrifuging.

B.決定 手順：

1. Preheat the spectrophotometer/microplate reader for 30min, 波長を調整して 450 nm, and set the spectrophotometer counter to zero with distilled
2. 決定:

C.計算：

96 井戸平底プレート

DBIL content (μmol/L) =1495.7×(ΔAT-ΔAB)-15.343

Micro glass キュベット

DBIL content (μmol/L) =1099.7×(ΔAT-ΔAB)-10.73

注記:

1. Bilirubin decomposes easily in light. Avoid light during
2. If the ΔA is lower, it is recommended to increase the sample size before determination; If ΔA＞5, 決定前にサンプルを希釈することをお勧めします.

例:

1. Take mouse serum to follow the determination procedure to operate. Determination with 96 井戸平底プレート, and calculate ΔAT=A1T-A2T=0.217-0.170=0.047, ΔAB=A1B-A2B=0.044-0.044=0. The calculated content is as follows:

DBIL content (μmol/L) =1495.7×(ΔAT-ΔAB)-15.343=54.95 μmol/L.

関連製品

BC5180/BC5185 Total bilirubin (TBIL) コンテンツアッセイキット