HiPure SF Plant DNA 96 キット

This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 分. DNA は次の目的に直接使用できます。 PCR, 定量的PCR, southern Blot, ウイルスのDNA検査など.

詳細

仕様

原理

Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

利点

• High quality DNA – meet a variety of downstream applications, PCRを含む, qPCR, 酵素消化, blot hybridization, 等.
• 高スループット – 96 samples can be processed simultaneously by 96 ウェルプレート
• High permeability – using composite membrane technology, no hole plugging

キット内容

保管と安定性

RNase solution should be stored at 2-8°C upon arrival. しかし, 短期保管 (まで 24 週) 室温で (15-25℃) パフォーマンスには影響しません. 残りのキットコンポーネントは乾燥した状態で室温で保管できます。 (15-25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月.

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