導入

The HiPure Plant RNA Midi Kit provides fast purification of high-quality RNA from plants, 細胞, 組織, and yeast using silica-membrane spin columns with a binding capacity of 1mg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, ノーザンブロット, ポリA+ RNA (mRNA) 精製, ヌクレアーゼ保護, およびin vitro翻訳.

詳細

仕様

原理

The Kit isolates total RNA from up to 1g plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 40 分. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 400µl water using the Kit.

利点

• DNAの効率的な除去 – DNase処理を伴わないユニークなゲノムDNA除去カラム
• 高品質 – 高純度総RNAは、さまざまな敏感な下流のアプリケーションで直接使用できます
• 速い – medium isolation of several samples can be completed in 40 分
• 安全 – フェノールクロロホルム抽出は必要ありません
• センシティブ – RNAはPgのレベルで回収できます
• Broad spectrum – various types of plant samples can be processed by diversity of operating procedures

キット内容

保管と安定性

キットは室温で乾燥させることができます (15-25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. 使用するときはすべてのバッファーが室温であることを確認してください. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.