HiPure HP 植物 RNA ミニキット

HiPure HP Plant RNA Mini Kit combines グアニジン isothiocyanate lysing and silica gel membrane purification technology to simplify total RNA抽出. Ultracentrifugation in CsCl purification and LiCl / ethanol precipitation are not required. The kit uses DNase digestion to completely remove DNA. It is suitable for extracting up to 100μg of total RNA (miRNAを含む) from plant samples less than 200mg. Several samples can be extracted within 40 分. The purified RNA can be directly used for RT-PCR, fluorescent quantitative RT-PCR, 北ハイブリダイゼーション, 第2世代シーケンス, 等.

詳細

仕様

原理

This kit uses glass fiber filter membrane purification technique, and only requires simple combination-washing-elution steps. The sample is lysed and homogenized in the solution containing guanidine salt, ethanol is added to provide appropriate binding conditions, and transferred to the purification column for centrifugation. Up to 100µg of RNA can be selectively bound to the membrane, pollutants are efficiently washed off after three times of washing, and finally the purified RNA is eluted by RNase Free Water.

利点

• Completely remove DNA by using of DNase
• 高品質 – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• 速い – いくつかのサンプルを抽出できます 40 列ごとの議事録
• センシティブ – RNAはPgのレベルで回収できます
• Broad spectrum – various types of plant samples can be processed by diversity of operating procedures

キット内容

保管と安定性

DNase I should be stored at -20-8°C upon arrival. 残りのキットコンポーネントは室温で保存できます (15-25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. キット全体は 2 ～ 8°C で保存できます。, ただし、この場合、バッファーは使用前に再溶解する必要があります。. 使用するときはすべてのバッファーが室温であることを確認してください.