HiPure Gel DNA Micro Kit

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, 順序付け, 制限消化, or various labeling reactions. 加えて, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

詳細

仕様

原理

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

利点

• High recovery efficiency – まで 80% DNA recovery
• Low elution volume – elution volume can be as low as 10μl
• 一般的な – recover DNA fromgel or enzyme-driven reaction solutions such as PCR
• Fast – isolation can be completed in 10-15 minutes by column gel method

キット内容

保管と安定性

The Kit should be stored dry at room temperature (15-25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. バッファー内に沈殿物が形成された場合, 37℃に温めて溶かす.