HiPure フルーツ RNA キット

The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, 細胞, 組織, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, ノーザンブロット, ポリA+ RNA(mRNA) 精製, nuclease protection and in vitro translation.

詳細

仕様

原理

The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 分. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.

利点

• Efficient DNA removal – one step RNA抽出 can effectively remove genomic DNA
• 高品質 – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• 速い – いくつかのサンプルを抽出できます 15 列ごとの議事録
• Wide range – samples including animals, 植物, 細菌, 細胞, 等
• High output – large sample amount, and the yield can be up to 200μg

キット内容

保管と安定性

キットは室温で乾燥させることができます (15–25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.