導入
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA スピンカラム to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
詳細
仕様
| 特徴 | 仕様 |
| 主な機能 | Co-isolation DNA and RNA from a single sample (細胞, soft tissue, plant sample) |
| アプリケーション | RT-PCR, cDNA合成, PCR andsecond-generation sequencing, 等. |
| 精製方法 | ミニスピンコラム |
| 精製技術 | シリカ技術 |
| 加工方法 | マニュアル (遠心分離または真空) |
| サンプルの種類 | Soft tissue samples (viscera, excluding skin andmuscle), cultured cells and common plant tissues |
| サンプル量 | Soft Tissue: < 30mg, 細胞: 1 バツ 107, 植物: 50 – 100mg |
| 収率 | DNA: 2 – 20 μg, RNA: 5 – 50 μg |
原理
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
利点
- 高品質 – 高純度総RNA / DNA can be directly used in a variety of downstream applications
- 速い – column method can complete the extraction of several samples in 30 分
- 安全 – フェノールクロロホルム抽出は必要ありません
- Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
キット内容
| コンテンツ | R511102 | R511103 |
| 浄化時間 | 50 準備 | 250 準備 |
| HiPure DNA Mini Columns | 50 | 250 |
| Hipure RNAミニ柱 | 50 | 250 |
| 2ml 採取管 | 100 | 2 バツ 250 |
| Buffer RLC | 50 ミリリットル | 200 ミリリットル |
| バッファDW1 | 30 ミリリットル | 150 ミリリットル |
| バッファRW1 | 30 ミリリットル | 150 ミリリットル |
| バッファRW2* | 20 ミリリットル | 2 バツ 50 ミリリットル |
| RNaseフリーウォーター | 10 ミリリットル | 30 ミリリットル |
| バッファAE | 10 ミリリットル | 50 ミリリットル |
保管と安定性
HiPure DNA/RNA Kit can be stored dry at room temperature (15–25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月.
レビュー
まだレビューはありません.