HiPure DNA Clean Up Kit

HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, 順序付け,制限消化, or various labeling reactions. 加えて, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.

詳細

仕様

原理

The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

利点

• High recovery efficiency – まで 80% DNA recovery
• 速い – isolation can be completed in 10 minutes by column gel method
• Low elution volume – elution volume can be as low as 10μl
• 効率的 – efficient removal of various inhibitory factors

キット内容

保管と安定性

HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. バッファー内に沈殿物が形成された場合, 37℃に温めて溶かす.