循環核酸マイクロキット

循環核酸マイクロキット, is designed to efficiently purify short nucleic acids (<1000血圧) from human biological fluids.

• Target Molecules:
  • Tumor-derived cell-free DNA fragments (cfDNA) and mRNA in blood
  • Fetal nucleic acids in maternal blood
  • Free-circulating cell-free RNA (CFRNA)
  • Viral nucleic acids
• Sample Compatibility:
  • Human plasma
  • Human serum
  • Human urine
  • Samples can be fresh or frozen (once only)
• Efficient Purification: The kit isolates short nucleic acids present in biofluids.
• 高純度: Purified nucleic acids are free of proteins, nucleases, その他の汚染物質.
• ダウンストリームアプリケーション: The eluted nucleic acids are ready for use in various downstream analyses, 含む:
  • Amplification reactions (例えば, PCR)
  • Long-term storage (-30°C to -15°C)

利点:

• Suitable for Short Nucleic Acids: Specifically designed to capture short fragments often found in biofluids.
• Broad Sample Compatibility: Works effectively with various human biofluids.
• High-Quality Results: Delivers purified nucleic acids free of impurities, ensuring reliable downstream applications.
• Convenient Storage: Purified nucleic acids can be stored for long-term use.

This kit empowers researchers studying circulating nucleic acids in various fields, including oncology, non-invasive prenatal testing (スキュー), and viral diagnostics.

仕様

特徴	仕様
主な機能	Isolation circulating DNA from 0.6ml plasma, 血清, 体液
アプリケーション	qPCR, liquid or solid chip analysis, ハイブリダイゼーション, and SNP detection, 等.
精製方法	ミニ スピンカラム
精製技術	シリカ技術
加工方法	マニュアル (遠心分離または真空)
サンプルの種類	血清, プラズマ, and other cell-free fluid samples
サンプル量	0.6ミリリットル
溶出量	≥30μL
実行あたりの時間	≤40 minutes
カラムあたりの液体搬送量	800μl
カラムの結合収量	100μg

原理

This product offers a fast and efficient method for purifying DNA using silica column chromatography. Here’s a breakdown of the process:

1. 溶解と消化:

• The sample is treated with a lysate solution and protease to break open cells and degrade proteins. This releases DNA into the solution.

2. Binding to Silica Membrane:

• The lysate containing the DNA is transferred to an adsorption plate or filtration column. The silica membrane in these tools selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.

3. 洗浄:

• Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.

4. 溶出:

• A low-salt buffer is used to elute the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing collection of purified DNA in a separate tube.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis, such as PCR, サザンブロット, および次世代シーケンス.

利点

• Efficient Purification: Silica column technology offers a streamlined process for isolating DNA from complex biological samples.
• High-Quality DNA: The selective binding properties of the silica membrane ensure the purification of high-purity DNA, suitable for various downstream applications.
• 簡素化されたワークフロー: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.

キット内容

コンテンツ	D318002	D318003
浄化時間	50 準備	250 準備
Buffer ACL	40 ミリリットル	200 ミリリットル
Buffer DCW1	22 ミリリットル	110 ミリリットル
Buffer DCW2*	20 ミリリットル	2 バツ 50 ミリリットル
プロテイナーゼK	34 mg	180 mg
プロテアーゼ溶解バッファー	1.8 ミリリットル	10 ミリリットル
キャリアRNA	110 μg	310 μg
Nuclease Free Water	10 ミリリットル	30 ミリリットル
HiPure CFDNA Mini Columns	50	250
2 ml 採取管	100	5 バツ 100

保管と安定性

Optimal Performance:

• Store Proteinase K and Carrier RNA at 2-8°C (冷蔵庫) upon arrival for best results.

許容可能な短期ストレージ:

• Proteinase K and Carrier RNA can be stored for up to 12 weeks at room temperature (15-25℃) without affecting their performance.

残りのキットコンポーネント:

• Store all other components of the kit dry at room temperature (15-25℃).
• They are stable for at least 18 この状況下で数か月.

重要なメモ:

• キット全体を2〜8°Cで保存する場合, ensure buffers are redissolved before use.
• Always warm all buffers to room temperature before using them for optimal performance.

By following these storage guidelines, you can ensure the longevity and effectiveness of your DNA extraction kit.