This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, プラズマ, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, ハイブリダイゼーション, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
詳細
仕様
| 特徴 | 仕様 |
| 主な機能 | 循環DNA/RNAの両方を分離 (miRNAを含めます) から 1-5 mlの血清と血漿 |
| アプリケーション | qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection |
| 精製方法 | ミディ スピンカラム |
| 精製技術 | シリカ技術, DNA filtration technology |
| 加工方法 | マニュアル (遠心分離または真空) |
| サンプルの種類 | 血清, プラズマ, and other cell-free liquid samples |
| サンプル量 | 1-5 ミリリットル |
| 溶出量 | ≥20μL |
| 実行あたりの時間 | ≤100 minutes |
| カラムあたりの液体搬送量 | 4 ミリリットル |
| カラムの結合収量 | 1 mg |
原理
This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. DNA / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. ついに, DNA / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection.
利点
- 高収量 – most optimized process to obtain maximum free RNA and small RNA
- 高濃度 – low elution volume (>20μl) to ensure nucleic acid concentration
- 高純度 – low alcohol combination, completely remove inhibitor and protein pollution
- 高い回復力 – silica gel column technology can recover nucleic acid molecules at the level of PG
- Large volume – 1-2ml serum and plasma samples can be processed at one time
キット内容
| コンテンツ | R431602 | D431603 |
| 浄化時間 | 50 準備 | 250 準備 |
| HiPure RNA Micro Columns | 50 | 5 バツ 50 |
| HiPure Viral Midi Columns | 50 | 5 バツ 50 |
| 15 ml 採取管 | 50 | 5 バツ 50 |
| 2ml 採取管 | 50 | 5 バツ 50 |
| Buffer CFL | 150 ミリリットル | 2 バツ 375 ミリリットル |
| Buffer CFP | 30 ミリリットル | 150 ミリリットル |
| Buffer MGW1* | 100 ミリリットル | 2 バツ 250 ミリリットル |
| バッファRW2* | 2 バツ 50 ミリリットル | 5 バツ 100 ミリリットル |
| RNaseフリーウォーター | 10 ミリリットル | 50 ミリリットル |
保管と安定性
The kit components can be stored at room temperature (15–25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月.
レビュー
まだレビューはありません.