HIPURE循環DNA MIDIキットc (COTプロトコル)

HiPure Circulating DNA Midi Kit, designed for efficient purification of short, cell-free DNA (cfDNA) molecules from human biofluids for clinical use:

• ターゲット:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Sample Compatibility:
  • Human plasma
  • Human serum
  • Human urine
  • Samples can be fresh or frozen (once only)
• Focus on Short DNA: The kit isolates short nucleic acids (<1000血圧) commonly found in biofluids.
• Efficient Purification: The protocol allows for effective recovery of cfDNA from various biofluids.
• Clinical Applications: The purified cfDNA is suitable for use in in vitro diagnostic (IVD) tests approved for clinical purposes.

利点:

• Optimized for Short cfDNA: Specifically designed to capture short DNA fragments relevant for biomarker discovery and liquid biopsies.
• Broad Sample Compatibility: Works effectively with various human biofluids, offering flexibility in sample selection.
• Larger Scale Purification: The Midi Kit format allows for processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
• Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.

This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (スキュー), and liquid biopsy analyses, ultimately aiding in patient diagnosis and management.

仕様

原理

This product offers a fast and reliable method for purifying DNA using silica column chromatography.

1. 溶解と消化: The sample is treated with a solution and enzyme to break open cells and degrade proteins, releasing DNA.
2. Binding to Silica: The lysate is transferred to a column. The silica membrane in the column captures DNA molecules, while impurities pass through.
3. 洗浄: Wash buffers to remove unbound proteins and cellular debris.
4. 溶出: A low-salt buffer elutes the purified DNA from the silica.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.

利点

• 高収量 – most optimal process, free DNA (>50血圧) can be obtained to the maximum extent
• 高濃度 – low elution volume, ensuring high nucleic acid concentration
• 高純度 – low alcohol binding method, completely removing inhibitor and protein pollution
• 高い回復力 – DNA can be recovered at the level of PG by silica gel column purification

キット内容

保管と安定性

• Keep it cool: Store Proteinase K and Carrier RNA in the fridge (2-8℃) for best results.
• Room temp is okay (for a while): They can last at room temperature (15-25℃) までのために 12 weeks without issues.
• Everything else: Store other kit components dry at room temperature (15-25℃), good for at least 18 月.
• Important: If storing everything cold, remember to warm up the buffers before use.

実験データ