導入
本製品は、厳格な選別と品質管理を経た核酸抽出用の中核原料です。. Guanidine thiocyanate is commonly used as lysate denaturant, and agarose gel sol in the process of nucleic acid extraction. Large 2m guanidine isothiocyanate can mediate the adsorption of isodna on glass fiber filter membranes or silica magnetic beads. Different from guanidine hydrochloride, guanidine isothiocyanate has a high absorbance value in A230, 0.5% (w / V) guanidine isothiocyanate (~4mm), and its A260 / A230 is less than 0.5. For the nucleic acid obtained by using this lysate, its A260 / 230 will be poor in low-concentration nucleic acid samples, but it will not affect PCR, RT-PCR, and second-generation sequencing.
詳細
仕様
| 特徴 | 仕様 | |
| 基本的な内容 | 推奨アプリケーション | アガロースゲル DNA精製, DNA/RNA分離 (溶解バッファー) |
| CAS番号 | 593-84-0 | |
| 式 | CH5N3•HSCN | |
| 分子量 | 118.6 | |
| コンテンツ | 99.5% | |
| レベル | 分子生物学 | |
| 外観 | White powder to crystal | |
| 輸送条件 | 室温, ドライ | |
| 保存条件 | Avoid light | |
| 不純物パラメータ | 水分 | ≤0.3% |
| Ash | ≤0.02% | |
| 水不溶性物質 | ≤0.02% | |
| Ammonium | ≤0.05% | |
| Ammonium dicyanide | ≤0.1％ | |
| Ammonium cyanuric chloride | ≤0.01％ | |
| 鉄 | ≤0.1PPM | |
| 紫外線吸収値 | 吸光度@ 230 (6M) | ≤20 |
| 吸光度@ 260 (6M) | ≤25 | |
| 吸光度@ 280 (6M) | ≤0.3 | |
| 吸光度@ 320 (6M) | ≤0.01 | |
| 核酸抽出関連 | Gel DNA Purification | 採用する |
| RNAの分離 | 採用する | |
| DNase テスト (1M) | 検出されず | |
| RNase検査 (1M) | 検出されず | |
| 飽和溶液の純度 (6M) | Endothermic during dissolution, no precipitation at room temperature after heating and dissolution, clear and colorless (no filtration) | |
| pH値 (1M) | 5.0-7.0 |
注文情報
| カタログ番号. | 商品名 | パッケージ |
| C11103 | Guanidine Thiocyanate
(分子生物学) |
5KG |
| C11104 | 25KG (5KG/Bag, 5Bags/Bucket) |
レビュー
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