導入
- Guanidine hydrochloride is a crucial component in nucleic acid extraction, selected after stringent screening and quality control.
- It acts as a powerful denaturant and ionizing agent, enhancing the solubility of hydrophobic molecules and denaturing proteins.
- Guanidine hydrochloride is effective in denaturing and inactivating various enzymes and proteins.
- で 6 M guanidine hydrochloride solution, the “secondary structure” of proteins, maintained by intermolecular non-covalent interactions, is disrupted, causing changes in the tertiary structure.
- It is commonly used in nucleic acid extraction as a cleaning solution (> 3M), binding solution (4-6M) for protein removal, and lysate for genomic DNA and total RNA抽出.
- Unlike guanidine isothiocyanate, guanidine hydrochloride exhibits low absorbance in A260, A280, and A230, resulting in an ideal ratio of nucleic acid product A260/A230.
仕様
| 特徴 | 仕様 | |
| 基本的な内容 | 推奨アプリケーション | プラスミド抽出, lysis Solution, or cleaning solution |
| CAS番号 | 50-01-1 | |
| 式 | CH5N3.HCL | |
| 分子量 | 95.53 | |
| コンテンツ | 99.5% | |
| レベル | 分子生物学 | |
| 外観 | White crystal will harden for a long time | |
| 輸送条件 | 室温 | |
| 保存条件 | 室温, ドライ, 暗い | |
| 不純物パラメータ | 水分 | ≤0.3% |
| Ash | ≤0.02% | |
| 水不溶性物質 | ≤0.02% | |
| Ammonium | ≤0.05% | |
| Ammonium dicyanide | ≤0.1％ | |
| Ammonium cyanuric chloride | ≤0.01％ | |
| 鉄 | ≤0.1PPM | |
| 紫外線吸収値 | 吸光度@ 230 (6M) | ≤0.4 |
| 吸光度@ 260 (6M) | ≤0.04 | |
| 吸光度@ 280 (6M) | ≤0.01 | |
| 吸光度@ 320 (6M) | ≤0.01 | |
| 核酸抽出関連 | プラスミド抽出試験 | 採用する |
| Blood DNA extraction test | 採用する | |
| DNase テスト (2M) | 検出されず | |
| RNase検査 (2M) | 検出されず | |
| 飽和溶液の純度 (8M) | The 8m mother liquor is heated and dissolved. After dissolution, the insoluble matter is filtered with 3 layers of rapid filter paper. | |
| pH値 (6M) | 5.0-5.5 |
注文情報
| カタログ番号. | 商品名 | パッケージ |
| C11101 | Guanidine Hydrochloride
(分子生物学) |
5KG |
| C11102 | 25KG (5KG/Bag, 5Bags/Bucket) |
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