Kit per il test dell'acido nucleico del virus della sindrome dei punti bianchi (WSSV). (Metodo con sonda fluorescente PCR) 24T

Questo prodotto deve essere imballato con sacche di ghiaccio e spedito tramite FedEx o UPS.
Arriverà all'interno 7-10 giorni. La commissione di spedizione è inclusa nel prezzo del prodotto.

Introduzione al prodotto：

This test kit offers a rapid and accurate method for detecting White Spot Syndrome Virus (WSSV) in shrimp.

• Tecnologia: Employs real-time fluorescent quantitative PCR (TaqMan probe method) for sensitive and specific detection.
• Targets: Utilizes specific primers and fluorescent probes designed to target the unique nucleic acid sequence of WSSV.
• Applicazioni: Detects WSSV in various shrimp samples, Compreso:
  • Animal tissues
  • Feed
  • Other sample types (consult kit specifications)
• Dual Detection: Provides qualitative and quantitative results:
  • Qualitative: Determines the presence or absence of WSSV.
  • Quantitative: Measures the amount of WSSV nucleic acid present, allowing for assessment of viral load.
• Real-Time Monitoring: Utilizes real-time PCR for continuous monitoring of amplification throughout the reaction, enabling rapid results.

This user-friendly test kit provides a valuable tool for shrimp farmers, researchers, and diagnostic labs for effective WSSV detection and management.

Contenuto del prodotto：

Componenti	WP2101005-01（24T）
WSSV reaction solution	23μL per well × 8 wells per strip × 3 strisce
WSSV positive control	100μL
controllo negativo	100μL

Magazzinaggio:

Conservare a -20 ° C., with a shelf life of at least 12 mesi.

Applicable instrument：

ABI series (ABI 7300/ABI 7500/Step One, eccetera.), Roche LightCycler series, Bio-Rad CFX 96, SanshiBio Q162D, and other real-time fluorescence quantitative PCR instruments

Elaborazione del campione：

Process the samples according to relevant standards, and store the processed samples for later use.

Experimental procedures：

• Preparazione del campione (Area di preparazione del campione)

Please refer to the instruction manual of the total DNA/Kit di estrazione dell'RNA, or use the ES08 fully automated nucleic acid extraction and purification instrument with a matching rapid nucleic acid extraction kit (magnetic bead method), or other nucleic acid extraction kits/methods that comply with relevant standards to extract nucleic acid from the processed samples. The extracted sample nucleic acid should be tested as soon as possible and stored in an icebox, or else stored at -20°C.

• Preparazione del sistema di reazione (Adding Area)

2.1 Take out the WSSV positive control tube, negative control tube, and reaction tubes containing the WSSV reaction solution required for the experiment (N+2) (i.e., n samples to be tested + controllo positivo + controllo negativo), completely thaw the reagents at room temperature, centrifuga per 10 secondi, centrifuge the liquid from the tube wall and lid to the bottom of the tube, and store in an icebox for later use.

2.2 Then add 2μL of extracted negative control, sample nucleic acid, and WSSV positive control to the reaction tube in the eight-row strip, close the tube lid, fare un record, and make the total volume of each reaction 25μL. Mescolare accuratamente, centrifuga per 30 secondi, and perform the amplification experiment on the real-time fluorescence quantitative PCR instrument.

• Fluorescent PCR Amplification (Area di amplificazione e analisi del prodotto)

Pre-denaturazione a 95 ° C per 3 minuti; denaturation at 95°C for 5 secondi, ricottura ed estensione a 60 ° C per 30 secondi, per 40 cicli, collect fluorescence signal at 60°C. Choose FAM as the fluorescence group, and None as the quencher group (for use with ABI series real-time fluorescence quantitative PCR instruments, se necessario, contact the manufacturer in advance or add ROX calibration dye by yourself, and select ROX as the quencher group; Altrimenti, Seguire la normale procedura).

• Result Determination

4.1 Ct value of the positive control < 30 e un “S” shaped amplification curve appears, the test result is valid. If not, L'esperimento dovrebbe essere ripetuto. If the retest is still invalid, Si prega di contattare il personale tecnico.

4.2 Sample detection result: Valore CT ≤ 38 e un “S” shaped amplification curve appears, indicating a positive result, which means the sample contains White Spot Syndrome Virus (WSSV).

4.3 Sample detection result: 38 < Ct < 40, it is considered suspicious. The sample should be retested. If the retest Ct value < 40 and there is a clear amplification curve, è considerato positivo; Altrimenti, è considerato negativo.

4.4 Sample detection result: Nessun valore CT o valore CT ≥ 40 e no “S” curva di amplificazione a forma di sagoma, è considerato negativo, indicating that White Spot Syndrome Virus (WSSV) was not detected in the sample.

Precauzioni：

• This test kit has high sensitivity. Per prevenire la contaminazione, the experiment needs to be strictly partitioned, and physical isolation between partitions is recommended to avoid cross-contamination caused by human factors.
• Indossare cappotti da laboratorio e guanti in lattice durante l'esperimento. Use separate tools in different areas, and remember to change gloves and lab coats.
• The components in the reaction solution are light-sensitive and should be stored away from light. Scongelare i reagenti completamente prima dell'uso, ma evita di congelare e scongelare ripetuti.
• Follow the instructions strictly for steps such as reagent preparation and sample addition. Disinfect the workbench, centrifuga, and pipettor regularly using chlorine disinfectants, etanolo, agenti di decontaminazione dell'acido nucleico, or ultraviolet light.
• Dopo la reazione, place the PCR reaction tubes in sealed bags for disposal. Do not open the tubes to avoid aerosol contamination.
• Do not mix reagents from different batches. Use within the expiration date. Consumables should be treated without enzymes and sterile.
• A negative test result does not completely indicate a non-infected state; it is closely related to the quality of the obtained nucleic acid. “Negativo” results may be due to unqualified nucleic acid sample quality, low sample nucleic acid load, or unsuccessful nucleic acid extraction (rilevamento). Veterinary clinical considerations are necessary for specific diagnoses.
• Possible causes of false positives: Cross-contamination during sample collection, transportation, and nucleic acid extraction.
• For any other questions, please contact the manufacturer’s technical staff promptly. Questo prodotto è solo per uso di ricerca scientifica e non è destinato alla diagnosi clinica o ad altri scopi.